Inter Simple Sequence Repeat Markers Associated with Flowering Time Duration in the Chilean Strawberry (<i>Fragaria chiloensis</i>)

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dc.contributor.authorCarrasco, B.
dc.contributor.authorRetamales, J. B.
dc.contributor.authorQuiroz, K.
dc.contributor.authorGarriga, M.
dc.contributor.authorCaligari, P. D. S.
dc.contributor.authorGarcia-Gonzales, R.
dc.date.accessioned2025-01-24T00:08:13Z
dc.date.available2025-01-24T00:08:13Z
dc.date.issued2013
dc.description.abstractThe flowering and fructification period of the Chilean strawberry (Fragaria chiloensis (L.) Duch.) is restricted to approximately 2 mo, which seriously limits the commercial development of the species. The objective of the current investigation was to identify Inter Simple Sequence Repeat (ISSR) polymorphisms associated with flowering duration in accessions of F. chiloensis. The flowering duration data related to 41 accessions obtained over 3 years were analyzed, and a set of 40 ISSR primers tested. Two clusters were obtained through the Partitioning Around Medoids algorithm, with 23 vs. 18 accessions, and 64.1 vs. 95.6 days of flowering, respectively. Flowering duration, between the two groups, was significantly different. The years also revealed a significant effect, on flowering duration, between the two groups. Ten of the ISSR primers tested revealed reproducible and consistent banding patterns, displaying a total of 106 putative loci, of which 79 were polymorphic. Three ISSR loci (811(779), 844(670), 841(980)) were identified owing to their significant contribution to the differentiation among the accessions. Similarly, three ISSR loci (811(600), 812(1180), 841(980)) exhibited a significant correlation with the flowering duration variation. Locus 841(980), which presented the highest level of correlation with flowering duration, was isolated, cloned and sequenced, but it showed only a low level of homology with the relevant sequences published in the GenBank database. The identified loci showing high correlation with the flowering time could help build Quantitative Trait Loci (QTL) maps for selection and improvement programs in the Fragaria sp. genus or other related species.
dc.description.funderPrograma Bicentenario de Ciencia y Tecnica (CONICYT-World Bank)
dc.fuente.origenWOS
dc.identifier.eissn2345-3737
dc.identifier.issn1680-7073
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101954
dc.identifier.wosidWOS:000327139000011
dc.issue.numero6
dc.language.isoen
dc.pagina.final1207
dc.pagina.inicio1195
dc.revistaJournal of agricultural science and technology
dc.rightsacceso restringido
dc.subjectAssociation analysis
dc.subjectISSR polymorphisms
dc.subjectMolecular markers
dc.subject.ods13 Climate Action
dc.subject.ods15 Life on Land
dc.subject.odspa13 Acción por el clima
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleInter Simple Sequence Repeat Markers Associated with Flowering Time Duration in the Chilean Strawberry (<i>Fragaria chiloensis</i>)
dc.typeartículo
dc.volumen15
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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