Social isolation of adolescent male rats increases anxiety and K<SUP>+</SUP>-induced dopamine release in the nucleus accumbens: Role of CRF-R1

Simple item page
dc.contributor.authorNovoa, Javier
dc.contributor.authorRivero, Carlos J.
dc.contributor.authorPerez-Cardona, Enrique U.
dc.contributor.authorFreire-Arvelo, Jaime A.
dc.contributor.authorZegers, Juan
dc.contributor.authorYarur, Hector E.
dc.contributor.authorSantiago-Marerro, Ivan G.
dc.contributor.authorAgosto-Rivera, Jose L.
dc.contributor.authorGonzalez-Perez, Jorge L.
dc.contributor.authorGysling, Katia
dc.contributor.authorSegarra, Annabell C.
dc.date.accessioned2025-01-20T22:16:08Z
dc.date.available2025-01-20T22:16:08Z
dc.date.issued2021
dc.description.abstractEarly life adversity can disrupt development leading to emotional and cognitive disorders. This study investigated the effects of social isolation after weaning on anxiety, body weight and locomotion, and on extracellular dopamine (DA) and glutamate (GLU) in the nucleus accumbens (NAc) and their modulation by corticotropin releasing factor receptor 1. On the day of weaning, male rats were housed singly or in groups for 10 consecutive days. Anxiety-like behaviors were assessed by an elevated plus maze (EPM) and an open field test (OF). Neurotransmitter levels were measured by in vivo microdialysis. Single-housed rats spent less time, and entered more, into the closed arms of an EPM than group-housed rats. They also spent less time in the center of an OF, weighed more and showed greater locomotion. In the NAc, no differences in CRF, or in basal extracellular DA or GLU between groups, were observed. A depolarizing stimulus increased DA release in both groups but to higher levels in isolated rats, whereas GLU increased only in single-housed rats. Blocking CRF-R1 receptors with CP-154,526 decreased DA release in single-housed but not in group-housed rats. The corticotropin releasing factor receptor type 1 receptor antagonist also decreased GLU in group-housed animals. These results show that isolating adolescent rats increases anxiety, body weight and ambulation, as well as the sensitivity of dopaminergic neurons to a depolarizing stimulus. This study provides further evidence of the detrimental effects of social isolation during early development and indicates that dysregulation of the CRF system in the NAc may contribute to the pathologies observed.
dc.fuente.origenWOS
dc.identifier.doi10.1111/ejn.15345
dc.identifier.eissn1460-9568
dc.identifier.issn0953-816X
dc.identifier.urihttps://doi.org/10.1111/ejn.15345
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94524
dc.identifier.wosidWOS:000670277000001
dc.issue.numero3
dc.language.isoen
dc.pagina.final4905
dc.pagina.inicio4888
dc.revistaEuropean journal of neuroscience
dc.rightsacceso restringido
dc.subjectCRF
dc.subjectDA
dc.subjectearly life stress
dc.subjectnucleus accumbens
dc.subjectyoung
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSocial isolation of adolescent male rats increases anxiety and K<SUP>+</SUP>-induced dopamine release in the nucleus accumbens: Role of CRF-R1
dc.typeartículo
dc.volumen54
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas