Chromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent

Simple item page
dc.contributor.authorMardones, G
dc.contributor.authorVenegas, A
dc.date.accessioned2025-01-21T01:31:24Z
dc.date.available2025-01-21T01:31:24Z
dc.date.issued2000
dc.description.abstractA solid agar plate assay was devised to discriminate bacteriolytic from bacteriostatic activity for a given antibacterial agent. The assay uses a bacterial culture harboring beta-galactosidase enzyme as reporter of cellular lysis. When a drop of bacteriolytic compound is placed on the agar, beta-galactosidase is released from the bacteria to the external solid medium where it hydrolyzes X-Gal substrate analogue, developing a blue halo at the edge of the inhibition growth zone. The assay was successfully evaluated against several antibiotics with well-known mechanism of action. It was found that bacteriostatic compounds consistently did not display blue halo at the inhibition zone. (C) 2000 Elsevier Science B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.issn0167-7012
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97057
dc.identifier.wosidWOS:000087279000001
dc.issue.numero3
dc.language.isoen
dc.pagina.final206
dc.pagina.inicio199
dc.revistaJournal of microbiological methods
dc.rightsacceso restringido
dc.subjectantibiotics
dc.subjectapidaecin
dc.subjectbacteriolytic assay
dc.subjectX-Gal plates
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleChromogenic plate assay distinguishing bacteriolytic from bacteriostatic activity of an antibiotic agent
dc.typeartículo
dc.volumen40
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas