Implementation and design of customized ex vivo machine perfusion. Analysis of its first results

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dc.contributor.authorRiveros, Sergio
dc.contributor.authorMarino, Carlo
dc.contributor.authorOchoa, Gabriela
dc.contributor.authorMorales, Emilio
dc.contributor.authorSoto, Dagoberto
dc.contributor.authorAlegria, Leyla
dc.contributor.authorJosefina Zenteno, Maria
dc.contributor.authorBranes, Alejandro
dc.contributor.authorAchurra, Pablo
dc.contributor.authorRebolledo, Rolando A.
dc.date.accessioned2024-01-10T12:37:05Z
dc.date.available2024-01-10T12:37:05Z
dc.date.issued2021
dc.description.abstractThe lack of organs available for transplantation is a global problem. The high mortality rates on the waiting list and the high number of discarded livers are reasons to develop new tools in the preservation and transplantation process. New tools should also be available for low-income countries. This article reports the development of customized normothermic machine perfusion (NMP). An ex vivo dual perfusion machine was designed, composed of a common reservoir organ box (CRO), a centrifugal pump (portal system, low pressure), and a roller pump (arterial system, high pressure). Porcine livers (n = 5) were perfused with an oxygenated normothermic (37celcius) strategy for 3 hours. Hemodynamic variables, metabolic parameters, and bile production during preservation were analyzed. Arterial and portal flow remain stable during perfusion. Total bilirubin production was 11.25 mL (4-14.5) at 180 minutes. The median pH value reached 7.32 (7.25-7.4) at 180 minutes. Lactate values decreased progressively to normalization at 120 minutes. This perfusion setup was stable and able to maintain the metabolic activity of a liver graft in a porcine animal model. Design and initial results from this customized NMP are promising for a future clinical application in low-income countries.
dc.description.funderChilean government support through the National Fund for Science and Technology (FONDECYT)
dc.fechaingreso.objetodigital2024-05-28
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1111/aor.14060
dc.identifier.eissn1525-1594
dc.identifier.issn0160-564X
dc.identifier.pubmedidMEDLINE:34519358
dc.identifier.urihttps://doi.org/10.1111/aor.14060
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76747
dc.identifier.wosidWOS:000696389900001
dc.information.autorucEscuela de medicina ; Riveros, Sergio ; S/I ; 178359
dc.information.autorucEscuela de medicina ; Marino, Carlo ; S/I ; 199753
dc.information.autorucEscuela de medicina ; Ochoa, Gabriela ; S/I ; 1083346
dc.information.autorucEscuela de medicina ; Morales, Emilio ; S/I ; 172100
dc.information.autorucEscuela de medicina ; Alegria, Leyla ; S/I ; 121090
dc.information.autorucEscuela de medicina ; Achurra, Pablo ; S/I ; 156236
dc.information.autorucInstituto de ingeniería biológica y médica ; Rebolledo, Rolando ; 0000-0003-3501-4042 ; 127520
dc.language.isoen
dc.nota.accesocontenido parcial
dc.publisherWiley
dc.revistaArtificial organs
dc.rightsacceso restringido
dc.subjectGraft preservation
dc.subjectLiver viability
dc.subjectMachine perfusion
dc.subjectOrgan preservation
dc.subjectTransplantation
dc.subjectLiver-Transplantation
dc.subjectPreservation
dc.subjectDonors
dc.subjectCold
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleImplementation and design of customized ex vivo machine perfusion. Analysis of its first results
dc.typeartículo
sipa.codpersvinculados178359
sipa.codpersvinculados199753
sipa.codpersvinculados1083346
sipa.codpersvinculados172100
sipa.codpersvinculados121090
sipa.codpersvinculados156236
sipa.codpersvinculados127520
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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