Dynamic changes in mRNA nucleocytoplasmic localization in the nitrate response of Arabidopsis roots

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dc.contributor.authorFonseca, Alejandro
dc.contributor.authorRiveras, Eleodoro
dc.contributor.authorMoyano, Tomas C.
dc.contributor.authorAlvarez, Jose M.
dc.contributor.authorRosa, Stefanie
dc.contributor.authorGutierrez, Rodrigo A.
dc.date.accessioned2025-01-20T16:14:02Z
dc.date.available2025-01-20T16:14:02Z
dc.date.issued2024
dc.description.abstractNitrate is a nutrient and signal that regulates gene expression. The nitrate response has been extensively characterized at the organism, organ, and cell-type-specific levels, but intracellular mRNA dynamics remain unexplored. To characterize nuclear and cytoplasmic transcriptome dynamics in response to nitrate, we performed a time-course expression analysis after nitrate treatment in isolated nuclei, cytoplasm, and whole roots. We identified 402 differentially localized transcripts (DLTs) in response to nitrate treatment. Induced DLT genes showed rapid and transient recruitment of the RNA polymerase II, together with an increase in the mRNA turnover rates. DLTs code for genes involved in metabolic processes, localization, and response to stimulus indicating DLTs include genes with relevant functions for the nitrate response that have not been previously identified. Using single-molecule RNA FISH, we observed early nuclear accumulation of the NITRATE REDUCTASE 1 (NIA1) transcripts in their transcription sites. We found that transcription of NIA1, a gene showing delayed cytoplasmic accumulation, is rapidly and transiently activated; however, its transcripts become unstable when they reach the cytoplasm. Our study reveals the dynamic localization of mRNAs between the nucleus and cytoplasm as an emerging feature in the temporal control of gene expression in response to nitrate treatment in Arabidopsis roots.
dc.description.abstractTranscriptome analysis of nuclear and cytoplasmic fractions in response to nitrate revealed transcripts with specific subcellular localization, revealing a novel layer of gene regulation. The dynamic interplay between mRNA localization, synthesis, and decay is crucial for the adaptive response of Arabidopsis roots to nitrate.
dc.fuente.origenWOS
dc.identifier.doi10.1111/pce.15018
dc.identifier.eissn1365-3040
dc.identifier.issn0140-7791
dc.identifier.urihttps://doi.org/10.1111/pce.15018
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90419
dc.identifier.wosidWOS:001258911800001
dc.issue.numero11
dc.language.isoen
dc.pagina.final4245
dc.pagina.inicio4227
dc.revistaPlant cell and environment
dc.rightsacceso restringido
dc.subjectgene expression regulation
dc.subjectnitrate
dc.subjectRNA dynamics
dc.subjectsubcellular localization
dc.subject.ods02 Zero Hunger
dc.subject.odspa02 Hambre cero
dc.titleDynamic changes in mRNA nucleocytoplasmic localization in the nitrate response of Arabidopsis roots
dc.typeartículo
dc.volumen47
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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