A network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception

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dc.contributor.authorRigal, Adeline
dc.contributor.authorDoyle, Siamsa M.
dc.contributor.authorRitter, Andres
dc.contributor.authorRaggi, Sara
dc.contributor.authorVain, Thomas
dc.contributor.authorO'Brien, Jose Antonio
dc.contributor.authorGoossens, Alain
dc.contributor.authorPauwels, Laurens
dc.contributor.authorRobert, Stephanie
dc.date.accessioned2025-01-20T22:08:19Z
dc.date.available2025-01-20T22:08:19Z
dc.date.issued2021
dc.description.abstractThe plant hormone auxin, a master coordinator of development, regulates hypocotyl elongation during seedling growth. We previously identified the synthetic molecule RubNeddin 1 (RN1), which induces degradation of the AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors INDOLE-3-ACETIC ACID-INDUCIBLE3 (IAA3) and IAA7 in planta and strongly promotes hypocotyl elongation. In the present study, we show that despite the structural similarity of RN1 to the synthetic auxin 2,4-dichlorophenoxyacetic-acid (2,4-D), direct treatments with these compounds in Arabidopsis (Arabidopsis thaliana) result in distinct effects, possibly due to enhanced uptake of RN1 and low-level, chronic release of 2,4-D from RN1 in planta. We confirm RN1-induced hypocotyl elongation occurs via specific TRANSPORT INHIBITOR RESISTANT1 (TIR1)/AUXIN SIGNALING F-BOX (AFB) receptor-mediated auxin signaling involving TIR1, AFB2, and AFB5. Using a transcriptome profiling strategy and candidate gene approach, we identify the genes ZINC FINGER OF ARABIDOPSIS THALIANA10 (ZAT10), ARABIDOPSIS TOXICOS EN LEVADURA31 (ATL31), and WRKY DNA-BINDING PROTEIN33 (WRKY33) as being rapidly upregulated by RN1, despite being downregulated by 2,4-D treatment. RN1-induced expression of these genes also occurs via TIR1/AFB-mediated auxin signaling. Our results suggest both hypocotyl elongation and transcription of these genes are induced by RN1 via the promoted degradation of the AUX/IAA transcriptional repressor IAA7. Moreover, these three genes, which are known to be stress-related, act in an inter-dependent transcriptional regulatory network controlling hypocotyl elongation. Together, our results suggest ZAT10, ATL31, and WRKY33 take part in a common gene network regulating hypocotyl elongation in Arabidopsis downstream of a selective auxin perception module likely involving TIR1, AFB2, and AFB5 and inducing the degradation of IAA7.
dc.fuente.origenWOS
dc.identifier.doi10.1093/plphys/kiab269
dc.identifier.eissn1532-2548
dc.identifier.issn0032-0889
dc.identifier.urihttps://doi.org/10.1093/plphys/kiab269
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94288
dc.identifier.wosidWOS:000696244400039
dc.issue.numero1
dc.language.isoen
dc.pagina.final445
dc.pagina.inicio430
dc.revistaPlant physiology
dc.rightsacceso restringido
dc.subject.ods02 Zero Hunger
dc.subject.ods13 Climate Action
dc.subject.odspa02 Hambre cero
dc.subject.odspa13 Acción por el clima
dc.titleA network of stress-related genes regulates hypocotyl elongation downstream of selective auxin perception
dc.typeartículo
dc.volumen187
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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