Molecular and population analyses of a recombination event in the catabolic plasmid pJP4

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dc.catalogadoraba
dc.contributor.authorLarraín-Linton, Juanita
dc.contributor.authorDe la Iglesia, R.
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorGonzález, B.
dc.date.accessioned2025-02-06T19:26:55Z
dc.date.available2025-02-06T19:26:55Z
dc.date.issued2006
dc.description.abstractCupriavidus necator JMP134(pJP4) harbors a catabolic plasmid, pJP4, which confers the ability to grow on chloroaromatic compounds. Repeated growth on 3-chlorobenzoate (3-CB) results in selection of a recombinant strain, which degrades 3-CB better but no longer grows on 2,4-dichlorophenoxyacetate (2,4-D). We have previously proposed that this phenotype is due to a double homologous recombination event between inverted repeats of the multicopies of this plasmid within the cell. One recombinant form of this plasmid (pJP4-F3) explains this phenotype, since it harbors two copies of the chlorocatechol degradation tfd gene clusters, which are essential to grow on 3-CB, but has lost the tfdA gene, encoding the first step in degradation of 2,4-D. The other recombinant plasmid (pJP4-FM) should harbor two copies of the tfdA gene but no copies of the tfd gene clusters. A molecular analysis using a multiplex PCR approach to distinguish the wild-type plasmid pJP4 from its two recombinant forms, was carried out. Expected PCR products confirming this recombination model were found and sequenced. Few recombinant plasmid forms in cultures grown in several carbon sources were detected. Kinetic studies indicated that cells containing the recombinant plasmid pJP4-FM were not selectable by sole carbon source growth pressure, whereas those cells harboring recombinant plasmid pJP4-F3 were selected upon growth on 3-CB. After 12 days of repeated growth on 3-CB, the complete plasmid population in C. necator JMP134 apparently corresponds to this form. However, wild-type plasmid forms could be recovered after growing this culture on 2,4-D, indicating that different plasmid forms can be found in C. necator JMP134 at the population level.
dc.fechaingreso.objetodigital2025-04-13
dc.format.extent9 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.1128/JB.00869-06
dc.identifier.eissn1098-5530
dc.identifier.issn0021-9193
dc.identifier.pubmedid16980481
dc.identifier.pubmedidPMC1595507
dc.identifier.scopusid2-s2.0-33749331862
dc.identifier.urihttps://doi.org/10.1128/JB.00869-06
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102182
dc.identifier.wosidWOS:000240711900008
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.issue.numeroNo. 19
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final6801
dc.pagina.inicio6793
dc.revistaJournal of Bacteriology
dc.rightsacceso abierto
dc.subject.ddc570
dc.subject.deweyBiología
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMolecular and population analyses of a recombination event in the catabolic plasmid pJP4
dc.typeartículo
dc.volumenVol. 188
sipa.codpersvinculados90468
sipa.codpersvinculados82342
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