SPERM BINDING TO THE HUMAN ZONA-PELLUCIDA AFTER MIGRATION THROUGH HUMAN CERVICAL-MUCUS

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dc.contributor.authorVIGIL, P
dc.contributor.authorRIQUELME, R
dc.contributor.authorMORALES, P
dc.date.accessioned2025-01-21T01:34:44Z
dc.date.available2025-01-21T01:34:44Z
dc.date.issued1995
dc.description.abstractDuring lactational amenorrhea a special type of cervical mucus, similar to that found during the luteal phase, is produced. This mucus, however, is able to support sperm migration. In the study described, the ability of spermatozoa to bind to the human zona pellucida (hZP) after migration through periovulatory and post-partum mucus was studied. Mucus was obtained from exclusively breastfeeding women in amenorrhea at 30, 60, 120 and 180 days post-partum. Periovulatory mucus samples from normally cycling women were used as a control. Flat capillary tubes were filled with BWW culture medium at the top and cervical mucus at the bottom. The tubes were immersed in a semen reservoir and the spermatozoa allowed to migrate through the mucus for 3 h into the culture media. Then the spermatozoa were coincubated with 3-4 hZP for 30 min and the number of bound spermatozoa per zona was counted. Periovulatory cervical mucus had an average Insler score of 14 +/- 0.5 as compared to 4.6 +/- 0.4 for post-partum mucus. Spermatozoa recovered from periovulatory mucus were always able to bind to the hZP. Spermatozoa recovered from post-partum mucus, however, were able to bind to the hZP in only 68 +/- 7% of the cases. Moreover, spermatozoa recovered from post-partum mucus bound to the ZP in lower numbers than did spermatozoa recovered from periovulatory mucus (p<0.03). These results suggest a greater ability of sperm-hZP binding after migration through periovulatory mucus and they also indicate that sperm binding to the ZP is possible even after sperm migration through a low quality mucus.
dc.fuente.origenWOS
dc.identifier.eissn1365-2605
dc.identifier.issn0105-6263
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97595
dc.identifier.wosidWOS:A1995RG84500003
dc.language.isoen
dc.pagina.final11
dc.pagina.inicio7
dc.revistaInternational journal of andrology
dc.rightsacceso restringido
dc.subjectBREAST-FEEDING
dc.subjectLACTATIONAL AMENORRHEA
dc.subjectPOSTPARTUM MUCUS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSPERM BINDING TO THE HUMAN ZONA-PELLUCIDA AFTER MIGRATION THROUGH HUMAN CERVICAL-MUCUS
dc.typeartículo
dc.volumen18
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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