Photodynamic inactivation strategies for maximizing antifungal effect against <i>Sporothrix</i> spp. and <i>Candida albicans</i> in an <i>in vitro</i> investigation

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dc.contributor.authorRocha, Amanda Regina
dc.contributor.authorInada, Natalia Mayumi
dc.contributor.authorda Silva, Ana Paula
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorBuzza, Hilde Harb
dc.date.accessioned2025-01-20T16:04:34Z
dc.date.available2025-01-20T16:04:34Z
dc.date.issued2024
dc.description.abstractBackground Sporotrichosis is a zoonotic disease caused by the dimorphic fungus Sporothrix spp., leading to skin lesions that can, in some cases, progress and result in the death of infected individuals. Candida albicans is another fungus involved in several skin, oral, and vaginal mucosal infections. Fungal diseases are concerning due to increasing incidence and the limited variety of antifungal classes available for treatment. Furthermore, antifungal medications can cause various side effects, exacerbated by their prolonged use during infection treatment. There is a need to explore alternatives to conventional drugs that are effective, fast, and safe in combating sporotrichosis. This study aimed to achieve in vitro elimination of the fungi Sporothrix brasiliensis and Sporothrix schenckii through Photodynamic Inactivation (PDI), using curcumin as a photosensitizer and in combination with antifungal agents used in the treatment of sporotrichosis.Methodology Yeasts of Candida albicans, Sporothrix brasiliensis, and Sporothrix schenckii were subjected to Photodynamic Inactivation (PDI) using light at a wavelength of 450 +/- 10 nm, irradiance of 35 mW/cm2, delivering a fluence of 31.5 J/cm2, with curcumin as the photosensitizer at doses ranging from 0.75 to 150 mu g/mL. After determining the Minimum Inhibitory Concentration (MIC) values of the antifungal drugs itraconazole, ketoconazole, and potassium iodide, sub-MIC doses of these antifungals were combined with sub-MIC doses of curcumin in a new PDI session.Conclusion Photodynamic inactivation is a promising technique in the treatment of sporotrichosis, as well as its combination with antifungals. The combination of curcumin in concentrations ranging from 0.75 g/mL a 7.5 g/mL with sub-MIC concentrations of itraconazole, ketoconazole, and potassium iodide was able to completely inactivate the fungi C. albicans, S. brasiliensis and S. schenckii, indicating that PDI may increase the effectiveness of antifungals. However, further studies are needed to establish protocols for future clinical applications.
dc.fuente.origenWOS
dc.identifier.doi10.1371/journal.pntd.0012637
dc.identifier.issn1935-2735
dc.identifier.urihttps://doi.org/10.1371/journal.pntd.0012637
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/89801
dc.identifier.wosidWOS:001352654700001
dc.issue.numero11
dc.language.isoen
dc.revistaPlos neglected tropical diseases
dc.rightsacceso restringido
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePhotodynamic inactivation strategies for maximizing antifungal effect against <i>Sporothrix</i> spp. and <i>Candida albicans</i> in an <i>in vitro</i> investigation
dc.typeartículo
dc.volumen18
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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