Membrane vesicles in <i>Acidithiobacillia</i> class extreme acidophiles: influence on collective behaviors of '<i>Fervidacidithiobacillus caldus</i>'

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dc.contributor.authorRossoni, Stefano
dc.contributor.authorBeard, Simon
dc.contributor.authorSegura-Bidermann, Maria Ignacia
dc.contributor.authorDuarte-Ramirez, Juan
dc.contributor.authorOsorio, Francisco Kirhman
dc.contributor.authorVaras-Godoy, Manuel
dc.contributor.authorMartinez-Bellange, Patricio
dc.contributor.authorVera, Mario
dc.contributor.authorQuatrini, Raquel
dc.contributor.authorCastro, Matias
dc.date.accessioned2025-01-20T17:09:09Z
dc.date.available2025-01-20T17:09:09Z
dc.date.issued2024
dc.description.abstractMembrane vesicles (MVs) are envelope-derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied in pathogenic microorganisms, the roles, relevance, and biotechnological potential of MVs from environmental bacteria are less well established. Acidithiobacillaceae family bacteria are active players in the sulfur and iron biogeochemical cycles in extremely acidic environments and drivers of the leaching of mineral ores contributing to acid rock/mine drainage (ARD/AMD) and industrial bioleaching. One key aspect of such a role is the ability of these bacteria to tightly interact with the mineral surfaces and extract electrons and nutrients to support their chemolithotrophic metabolism. Despite recent advances in the characterization of acidithiobacilli biofilms and extracellular matrix (ECM) components, our understanding of its architectural and mechanistic aspects remains scant. Using different microscopy techniques and nano-tracking analysis we show that vesiculation is a common phenomenon in distant members of the Acidithiobacillaceae family, and further explore the role of MVs in multicellular colonization behaviors using 'Fervidacidithiobacillus caldus' as a bacterial model. Production of MVs in 'F. caldus' occurred in both planktonic cultures and biofilms formed on sulfur surfaces, where MVs appeared individually or in chains resembling tube-shaped membranous structures (TSMSs) important for microbial communication. Liquid chromatography-mass spectrometry data and bioinformatic analysis of the MV-associated proteome revealed that 'F. caldus' MVs were enriched in proteins involved in cell-cell and cell-surface processes and largely typified the MVs as outer MVs (OMVs). Finally, microbiological assays showed that amendment of 'F. caldus' MVs to cells and/or biofilms affects collective colonizing behaviors relevant to the ecophysiology and applications of these acidophiles, providing grounds for their exploitation in biomining.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fmicb.2023.1331363
dc.identifier.eissn1664-302X
dc.identifier.urihttps://doi.org/10.3389/fmicb.2023.1331363
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91017
dc.identifier.wosidWOS:001158762300001
dc.language.isoen
dc.revistaFrontiers in microbiology
dc.rightsacceso restringido
dc.subjectouter membrane vesicles (OMVs)
dc.subjectAcidithiobacillus
dc.subjectsurface colonization
dc.subjectbiofilm
dc.subjectswarming
dc.subjectadhesins
dc.subjectsulfur oxidation
dc.subjecttube-shaped membranous structures (TSMS)
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMembrane vesicles in <i>Acidithiobacillia</i> class extreme acidophiles: influence on collective behaviors of '<i>Fervidacidithiobacillus caldus</i>'
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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