Genome sequencing and transcriptomic analysis of the Andean killifish Orestias ascotanensis reveals adaptation to high-altitude aquatic life

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dc.contributor.authorDi Genova, Alex
dc.contributor.authorNardocci, Gino
dc.contributor.authorMaldonado-Agurto, Rodrigo
dc.contributor.authorHodar, Christian
dc.contributor.authorValdivieso, Camilo
dc.contributor.authorMorales, Pamela
dc.contributor.authorGajardo, Felipe
dc.contributor.authorMarina, Raquel
dc.contributor.authorGutierrez, Rodrigo A.
dc.contributor.authorOrellana, Ariel
dc.contributor.authorCambiazo, Veronica
dc.contributor.authorGonzalez, Mauricio
dc.contributor.authorGlavic, Alvaro
dc.contributor.authorMendez, Marco A.
dc.contributor.authorMaass, Alejandro
dc.contributor.authorAllende, Miguel L.
dc.contributor.authorMontecino, Martin A.
dc.date.accessioned2025-01-20T22:01:22Z
dc.date.available2025-01-20T22:01:22Z
dc.date.issued2022
dc.description.abstractOrestias ascotanensis (Cyprinodontidae) is a teleost pupfish endemic to springs feeding into the Ascotan saltpan in the Chilean Altiplano (3,700 m.a.s.l.) and represents an opportunity to study adaptations to high-altitude aquatic environments. We have de novo assembled the genome of O. ascotanensis at high coverage. Comparative analysis of the O. ascotanensis genome showed an overall process of contraction, including loss of genes related to Gprotein signaling, chemotaxis and signal transduction, while there was expansion of gene families associated with microtubule-based movement and protein ubiquitination. We identified 818 genes under positive selection, many of which are involved in DNA repair. Additionally, we identified novel and conserved microRNAs expressed in O. ascotanensis and its closely-related species, Orestias gloriae. Our analysis suggests that positive selection and expansion of genes that preserve genome stability are a potential adaptive mechanism to cope with the increased solar UV radiation to which high-altitude animals are exposed to.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.ygeno.2021.12.018
dc.identifier.eissn1089-8646
dc.identifier.issn0888-7543
dc.identifier.urihttps://doi.org/10.1016/j.ygeno.2021.12.018
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93818
dc.identifier.wosidWOS:000742146100002
dc.issue.numero1
dc.language.isoen
dc.pagina.final315
dc.pagina.inicio305
dc.revistaGenomics
dc.rightsacceso restringido
dc.subjectOrestias ascotanensis
dc.subjectDNA repair
dc.subjectHigh-altitude
dc.subjectDesert pupfish
dc.subjectAltiplano
dc.subjectmicroRNAs
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGenome sequencing and transcriptomic analysis of the Andean killifish Orestias ascotanensis reveals adaptation to high-altitude aquatic life
dc.typeartículo
dc.volumen114
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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