An α-L-arabinofuranosidase from <i>Penicillium</i> <i>purpurogenum</i>

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dc.contributor.authorDe Ioannes, P
dc.contributor.authorPeirano, A
dc.contributor.authorSteiner, J
dc.contributor.authorEyzaguirre, J
dc.date.accessioned2025-01-21T01:31:37Z
dc.date.available2025-01-21T01:31:37Z
dc.date.issued2000
dc.description.abstractPenicillium purpurogenum secretes arabinofuranosidase to the growth medium. Highest levels of enzyme (1.0 U ml(-1)) are obtained when L-arabitol is used as carbon source, while 0.85 and 0.7 U ml(-1) are produced with sugar beet pulp and oat spelts xylan, respectively. By means of a zymogram, three bands with arabinofuranosidase activity have been detected in the supernatant of a culture grown in oat spelts xylan. One of the enzymes was purified to homogeneity from this supernatant using gel filtration (BioGel P-100), cation exchange chromatography (CM-Sephadex C-50), hydrophobic interaction chromatography (phenyl agarose) and a second BioGel P-100 column. The enzyme is a monomer of 58 kDa with a pI of 6.5. Optimum pH is 4.0 and optimal temperature 50 degrees C. The arabinofuranosidase is highly specific for alpha-L-arabinofuranosides and liberates arabinose from arabinoxylan. The enzyme shows hyperbolic kinetics towards p-nitrophenyl-alpha-L-arabinofuranoside with a K-M of 1.23 mM. A 36-residue N-terminal sequence is over 70% identical to that of fungal arabinofuranosidases belonging to family 54 of the glycosyl hydrolases. Based on the sequence similarity and other biochemical properties it is proposed that the purified enzyme from P. purpurogenum belongs to family 54. (C) 2000 Elsevier Science B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.eissn1873-4863
dc.identifier.issn0168-1656
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97105
dc.identifier.wosidWOS:000084886400015
dc.issue.numero2-3
dc.language.isoen
dc.pagina.final258
dc.pagina.inicio253
dc.revistaJournal of biotechnology
dc.rightsacceso restringido
dc.subjectPenicillium purpurogenum
dc.subjectarabinofuranosidase
dc.subjectglycosyl hydrolase family 54
dc.subjectenzyme purification
dc.subject.ods12 Responsible Consumption and Production
dc.subject.ods07 Affordable and Clean Energy
dc.subject.odspa12 Producción y consumo responsable
dc.subject.odspa07 Energía asequible y no contaminante
dc.titleAn α-L-arabinofuranosidase from <i>Penicillium</i> <i>purpurogenum</i>
dc.typeartículo
dc.volumen76
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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