LOCALIZATION OF MICROFILAMENTS AND A TUBULIN-LIKE PROTEIN IN CRUSTACEAN (RHYNCHOCINETES-TYPUS) SPERMATOZOON

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dc.contributor.authorPEREZ, C
dc.contributor.authorROCO, M
dc.contributor.authorCASTRO, A
dc.contributor.authorDUPRE, E
dc.contributor.authorSCHATTEN, G
dc.contributor.authorBARROS, C
dc.date.accessioned2025-01-23T19:22:42Z
dc.date.available2025-01-23T19:22:42Z
dc.date.issued1991
dc.description.abstractSperm from the decapod crustacean Rhynchocinetes typus undergo dramatic shape changes as they pass from the vas deferens to seawater and interact with the oocyte envelopes. Using FITC-phalloidin and antitubulin antibodies, we were able to localize microfilaments and a tubulin-like protein in R. typus spermatozoon. Microfilaments and the tubulin-like protein were associated with the sperm rays and spines, but were absent at the spike and at its base. Folded and unfolded spermatozoa display similar fluorescence patterns. SDS-PAGE of whole spermatozoa and electrotransfer to nitrocellulose confirmed the presence of actin and two proteins at 97 kd and 120 kd that bind to tubulin antibodies (tubulin-like proteins). These results demonstrate the presence of actin, but not tubulin, and localize microfilaments in these sperm. It is proposed that this cytoskeletal component is active in sperm during crustacean fertilization.
dc.fuente.origenWOS
dc.identifier.issn1040-452X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99085
dc.identifier.wosidWOS:A1991FD13300009
dc.issue.numero4
dc.language.isoen
dc.pagina.final379
dc.pagina.inicio373
dc.revistaMolecular reproduction and development
dc.rightsacceso restringido
dc.subjectSPERM
dc.subjectACTIN
dc.subjectTUBULIN-LIKE PROTEIN
dc.subject.ods14 Life Below Water
dc.subject.ods15 Life on Land
dc.subject.odspa14 Vida submarina
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleLOCALIZATION OF MICROFILAMENTS AND A TUBULIN-LIKE PROTEIN IN CRUSTACEAN (RHYNCHOCINETES-TYPUS) SPERMATOZOON
dc.typeartículo
dc.volumen28
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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