Microsatellite analysis of synchronous and metachronous tumors - A tool for double primary tumor and metastasis assessment

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dc.contributor.authorTang, MY
dc.contributor.authorPires, Y
dc.contributor.authorSchultz, M
dc.contributor.authorDuarte, I
dc.contributor.authorGallegos, M
dc.contributor.authorWistuba, II
dc.date.accessioned2025-01-21T01:09:03Z
dc.date.available2025-01-21T01:09:03Z
dc.date.issued2003
dc.description.abstractDespite well-established histopathological features and the development of immunostaining of human neoplasms, there are a number of cases in which surgical pathologists cannot assure the origin of synchronous and metachronous tumors. In many cases, the classification of these lesions as either two separate primary tumors or as a single primary tumor with a metastasis has significant implications with respect to patient prognosis and recommendations for therapy. To establish the origin of tumors, we assessed tumor cell clonality using PCR-based microsatellite analysis on microdissected archival tissues for loss of heterozygosity (LOH) and microsatellite instability (NISI) in a series of 19 paired synchronous and metachronous tumors from several organs. As a control group, 15 autopsy cases with an unequivocally recognizable primary tumor and associated metastases were also examined. Based on LOH and MSI findings, and using a panel of 4 to 12 (median 7) microsatellite markers, we were able to establish the clonal pattern of microsatellite changes in 17 out of 19 (89%) biopsy cases and thus determine if they were either double primary tumors (41%) or metastases (59%). Of interest, identical or similar pattern of microsatellite abnormalities were detected in 15 primary tumors and corresponding metastasis from autopsies. Our results indicate that microsatellite analysis for LOH and MSL as an expression of clonality, provides a useful tool to distinguish double primary neoplasms and metastases in synchronous and metachronous tumors.
dc.fuente.origenWOS
dc.identifier.eissn1533-4066
dc.identifier.issn1052-9551
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96540
dc.identifier.wosidWOS:000185019500006
dc.issue.numero3
dc.language.isoen
dc.pagina.final159
dc.pagina.inicio151
dc.revistaDiagnostic molecular pathology
dc.rightsacceso restringido
dc.subjectdouble tumors
dc.subjectmicrodissection
dc.subjectloss of heterozygosity
dc.subjectmicrosatellite instability
dc.subjectmolecular diagnosis
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMicrosatellite analysis of synchronous and metachronous tumors - A tool for double primary tumor and metastasis assessment
dc.typeartículo
dc.volumen12
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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