SUBUNITS OF YEAST RNA POLYMERASE-I INVOLVED IN INTERACTIONS WITH DNA AND NUCLEOTIDES

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dc.contributor.authorVALENZUELA, P
dc.contributor.authorBULL, P
dc.contributor.authorZALDIVAR, J
dc.contributor.authorVENEGAS, A
dc.contributor.authorMARTIAL, J
dc.date.accessioned2025-01-23T19:44:52Z
dc.date.available2025-01-23T19:44:52Z
dc.date.issued1978
dc.description.abstractReaction of yeast [Saccharomyces cerevisiae] RNA polymerase I with pyridoxal 5''-phosphate and sodium borohydride under conditions which inactivate the enzyme results in the specific binding of pyridoxal 5''-phosphate to subunits of 185,000, 137,000, 48,000 and 36,000 daltons. Nucleotides, which protect the enzyme from inactivation specifically inhibit the binding of pyridoxal 5''-phosphate to subunits of 185,000 and 137,000 daltons. DNA which also protects the enzyme from inactivation by pyridoxal 5''-phosphate prevents the binding of the reagent to the 4 polypeptides. These results suggest that subunits of 185,000 and 137,000 are involved in interactions with both nucleotides and DNA presumably of the type leading to initiation and/or polymerization and that subunits of 48,000 and 36,000 daltons also bind to DNA but this interaction is not strictly required for polymerase activity.
dc.fuente.origenWOS
dc.identifier.eissn1090-2104
dc.identifier.issn0006-291X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100147
dc.identifier.wosidWOS:A1978EV91500061
dc.issue.numero2
dc.language.isoen
dc.pagina.final666
dc.pagina.inicio662
dc.revistaBiochemical and biophysical research communications
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSUBUNITS OF YEAST RNA POLYMERASE-I INVOLVED IN INTERACTIONS WITH DNA AND NUCLEOTIDES
dc.typeartículo
dc.volumen81
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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