Trypsin-like enzymes during fertilization in the shrimp Rhynchocinetes typus

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dc.contributor.authorRios, M
dc.contributor.authorBarros, C
dc.date.accessioned2025-01-21T01:33:24Z
dc.date.available2025-01-21T01:33:24Z
dc.date.issued1997
dc.description.abstractThe spermatozoon of Rhynchocinetes typus is atypical because it is nonmotile and lacks head and tail. The body has a rigid spike. Neither an acrosome-like structure nor changes during gamete interaction which could be interpreted as an acrosome reaction have been observed in this species. Nevertheless, the spermatozoon exerts a lytic effect on the extracellular envelope of the egg, and in this way it penetrates through egg-coats, forming a channel. In this research we found that crude spermatozoa. extracts analyzed by gelatin SDS-PAGE developed one band of protease activity that was completely inhibited by SBTI (soybean trypsin inhibitor) and pAB (p-aminobenzamidine). In sperm extracts an enzymatic activity was determined, using BAEE (N-benzoil-L- arginine ethyl ester), but not ATEE, as substrate, This activity was inhibited by SBTI and pAB. We observed that in vitro fertilization was inhibited by spermatozoon incubation with the trypsin inhibitors SBTI, PMSF (phenyl-methanesulphonyl fluoride), and pAB. Additionally, we observed that when whole isolated egg-coats were incubated with sperm extract and then analyzed by SDS-PAGE, one band of the egg-coats disappeared, These results have been interpreted as sperm trypsinlike enzyme participation in R. typus sperm passage through the egg-coats. (C) 1997 Wiley-Liss, Inc.
dc.fuente.origenWOS
dc.identifier.issn1040-452X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97417
dc.identifier.wosidWOS:A1997WQ41900015
dc.issue.numero4
dc.language.isoen
dc.pagina.final586
dc.pagina.inicio581
dc.revistaMolecular reproduction and development
dc.rightsacceso restringido
dc.subjectsperm
dc.subjectegg-coat
dc.subjecttrypsin-like
dc.subjectshrimp
dc.subjectfertilization
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTrypsin-like enzymes during fertilization in the shrimp Rhynchocinetes typus
dc.typeartículo
dc.volumen46
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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