Differential Sars-cov-2 antigen specificity of the humoral response inactivated virus-vaccinated, convalescent, and breakthrough subjects

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dc.catalogadorvzp
dc.contributor.authorDuarte Peñaloza, Luisa Fernanda
dc.contributor.authorVázquez Hernández, Yaneisi
dc.contributor.authorDiethelm Varela, Benjamin Manuel
dc.contributor.authorPávez Carrasco, Valentina Ignacia
dc.contributor.authorBerrios Rojas, Roslye
dc.contributor.authorWhite, Jessica A.
dc.contributor.authorKalergis Parra, Alexis Mikes
dc.contributor.authorBueno Ramírez, Susan Marcela
dc.contributor.authorGonzalez Munoz, Pablo Alberto
dc.date.accessioned2024-01-30T13:46:11Z
dc.date.available2024-01-30T13:46:11Z
dc.date.issued2022
dc.description.abstractAnalytical methods for the differential determination between natural infection with SARS- CoV-2 vs. immunity elicited by vaccination or infection after immunization (breakthrough cases) represent attractive new research venues in the context of the ongoing COVID-19 pandemic caused by Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2). Herein, we set out to compare humoral responses against several SARS-CoV-2 structural and non-structural proteins in infected unvaccinated (convalescent), vaccinated, as well as vaccinated and infected (breakthrough) individuals. Our results indicate that immunization with an inactivated SARS-CoV-2 vaccine (CoronaVac) induces significantly higher levels of IgG antibodies against the membrane (M) protein of SARS-CoV-2 as compared to convalescent subjects both, after the primary vaccination schedule and after a booster dose. Moreover, we found that CoronaVac-immunized individuals, after receiving a third vaccine shot, display equivalent levels of N-specific IgG antibodies as convalescents subjects. Regarding non-structural viral proteins, for the two viral proteins ORF3a and NSP8, IgG antibodies were produced in more than 50% of the convalescent subjects. Finally, a logistic regression model and a receiver operating characteristic (ROC) analysis show that combined detection of M and N proteins may be useful as a biomarker to differentiate breakthrough cases from vaccinated and convalescent individuals that did not receive prior vaccination. Taken together, these results suggest that multiple SARS-CoV-2 antigens may be used as differential biomarkers for distinguishing natural infection from vaccination.
dc.fechaingreso.objetodigital2024-10-28
dc.format.extent41 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1101/2022.07.01.22277165
dc.identifier.urihttps://doi.org/10.1101/2022.07.01.22277165
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81051
dc.information.autorucFacultad de Ciencias Biológicas; Duarte Peñaloza Luisa Fernanda; 0000-0003-1828-1800; 1031205
dc.information.autorucFacultad de Ciencias Biológicas; Vazquez Hernandez Yaneisi; S/I; 1025308
dc.information.autorucFacultad de Ciencias Biológicas; Diethelm Varela Benjamin Manuel; 0000-0002-5465-6924; 217234
dc.information.autorucFacultad de Ciencias Biológicas; Pavez Carrasco Valentina Ignacia; S/I; 249608
dc.information.autorucFacultad de Ciencias Biológicas; Kalergis Parra Alexis Mikes; 0000-0001-7622-5263; 90610
dc.information.autorucFacultad de Ciencias Biológicas; Bueno Ramírez, Susan Marcela; 0000-0002-7551-8088; 113541
dc.information.autorucFacultad de Ciencias Biológicas; González Muñoz, Pablo Alberto; 0000-0001-7709-6870; 15616
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final41
dc.pagina.inicio1
dc.revistamedRxiv. The prerint server for health science
dc.rightsacceso restringido
dc.subjectInactivated Vaccine
dc.subjectCoronaVac
dc.subjectSARS-CoV-2
dc.subjectAntigens
dc.subjectDifferential diagnosis
dc.subjectBiomarkers
dc.subject.ddc610
dc.subject.deweyMedicina y salud
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferential Sars-cov-2 antigen specificity of the humoral response inactivated virus-vaccinated, convalescent, and breakthrough subjects
dc.typepreprint
sipa.codpersvinculados1031205
sipa.codpersvinculados1025308
sipa.codpersvinculados217234
sipa.codpersvinculados249608
sipa.codpersvinculados90610
sipa.codpersvinculados113541
sipa.codpersvinculados15616
sipa.trazabilidadORCID;2024-01-15
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