THE MAJOR THIOBACILLUS-FERROOXIDANS OUTER-MEMBRANE PROTEIN FORMS LOW CONDUCTANCE ION CHANNELS IN PLANAR LIPID BILAYERS

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dc.contributor.authorSILVA, M
dc.contributor.authorFERREIRA, A
dc.contributor.authorRODRIGUEZ, M
dc.contributor.authorWOLFF, D
dc.date.accessioned2025-01-23T19:22:21Z
dc.date.available2025-01-23T19:22:21Z
dc.date.issued1992
dc.description.abstractA protein isolated and purified from the outer membrane of the acidophilic, chemolithotrophic bacterium, Thiobacillus ferrooxidans with an oligomeric molecular weight of 90 000 Da (p90) was incorporated into phosphatidylethanolamine planar lipid bilayers. The protein formed slightly anionic channels in KCl solutions, with a conductance of 25 pS in 100 mM KCl. The current-voltage relationship was linear between +/-60 mV, and the conductance was a saturating function of the salt concentration. These channels fluctuated from a single open to closed state at low potentials, but present flickering activity at higher potentials.
dc.fuente.origenWOS
dc.identifier.issn0014-5793
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99020
dc.identifier.wosidWOS:A1992HA63700012
dc.issue.numero2
dc.language.isoen
dc.pagina.final173
dc.pagina.inicio169
dc.revistaFebs letters
dc.rightsacceso restringido
dc.subjectION CHANNEL
dc.subjectPORIN
dc.subjectLIPID BILAYER
dc.subjectAUTOTROPHIC BACTERIA
dc.subjectTHIOBACILLUS-FERROOXIDANS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTHE MAJOR THIOBACILLUS-FERROOXIDANS OUTER-MEMBRANE PROTEIN FORMS LOW CONDUCTANCE ION CHANNELS IN PLANAR LIPID BILAYERS
dc.typeartículo
dc.volumen296
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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