Dlg Is Required for Short-Term Memory and Interacts with NMDAR in the <i>Drosophila</i> Brain

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dc.contributor.authorBertin, Francisca
dc.contributor.authorMoya-Alvarado, Guillermo
dc.contributor.authorQuiroz-Manriquez, Eduardo
dc.contributor.authorIbacache, Andres
dc.contributor.authorKohler-Solis, Andres
dc.contributor.authorOliva, Carlos
dc.contributor.authorSierralta, Jimena
dc.date.accessioned2025-01-20T21:02:58Z
dc.date.available2025-01-20T21:02:58Z
dc.date.issued2022
dc.description.abstractThe vertebrates' scaffold proteins of the Dlg-MAGUK family are involved in the recruitment, clustering, and anchoring of glutamate receptors to the postsynaptic density, particularly the NMDA subtype glutamate-receptors (NRs), necessary for long-term memory and LTP. In Drosophila, the only gene of the subfamily generates two main products, dlgA, broadly expressed, and dlgS97, restricted to the nervous system. In the Drosophila brain, NRs are expressed in the adult brain and are involved in memory, however, the role of Dlg in these processes and its relationship with NRs has been scarcely explored. Here, we show that the dlg mutants display defects in short-term memory in the olfactory associative-learning paradigm. These defects are dependent on the presence of DlgS97 in the Mushroom Body (MB) synapses. Moreover, Dlg is immunoprecipitated with NRs in the adult brain. Dlg is also expressed in the larval neuromuscular junction (NMJ) pre and post-synaptically and is important for development and synaptic function, however, NR is absent in this synapse. Despite that, we found changes in the short-term plasticity paradigms in dlg mutant larval NMJ. Together our results show that larval NMJ and the adult brain relies on Dlg for short-term memory/plasticity, but the mechanisms differ in the two types of synapses.
dc.description.funderFondecyt
dc.fuente.origenWOS
dc.identifier.doi10.3390/ijms23169187
dc.identifier.eissn1422-0067
dc.identifier.urihttps://doi.org/10.3390/ijms23169187
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93109
dc.identifier.wosidWOS:000845576800001
dc.issue.numero16
dc.language.isoen
dc.revistaInternational journal of molecular sciences
dc.rightsacceso restringido
dc.subjectsynapse
dc.subjectDrosophila
dc.subjectshort-term memory
dc.subjectpost-tetanic potentiation
dc.subjectelectrophysiological
dc.subjectpost-tetanic depression
dc.subjectDlgS97
dc.subjectDlgA
dc.subjectneuromuscular junction
dc.subjectNMDAR
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDlg Is Required for Short-Term Memory and Interacts with NMDAR in the <i>Drosophila</i> Brain
dc.typeartículo
dc.volumen23
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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