Interferon-β decreases LPS-induced neutrophil recruitment to cardiac fibroblasts

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dc.contributor.authorAnfossi, Renatto
dc.contributor.authorVivar, Raul
dc.contributor.authorAyala, Pedro
dc.contributor.authorGonzalez-Herrera, Fabiola
dc.contributor.authorEspinoza-Perez, Claudio
dc.contributor.authorOsorio, Jose Miguel
dc.contributor.authorRoman-Torres, Mauricio
dc.contributor.authorBolivar, Samir
dc.contributor.authorDiaz-Araya, Guillermo
dc.date.accessioned2025-01-20T17:34:39Z
dc.date.available2025-01-20T17:34:39Z
dc.date.issued2023
dc.description.abstractIntroduction: Cardiac fibroblasts (CF) are crucial cells in damaged heart tissues, expressing TLR4, IFN-receptor and responding to lipopolysaccharide (LPS) and interferon-beta (IFN-beta) respectively. While CF interact with immune cells; however, their relationship with neutrophils remains understudied. Additionally, theimpact of LPS and IFN-beta on CF-neutrophil interaction is poorly understood.Methods: Isolated CF from adult rats were treated with LPS, with or without IFN-beta. This study examined IL-8 secretion, ICAM-1 and VCAM-1 expression, and neutrophil recruitment, as well as their effects on MMPs activity.Results: LPS triggered increased IL-8 expression and secretion, along with elevated ICAM-1 and VCAM-1 expression, all of which were blocked by TAK-242. Pre-treatment with IFN-beta countered these LPS effects. LPS treated CF showed higher neutrophil recruitment (migration and adhesion) compared to unstimulated CF, an effect prevented by IFN-beta. Ruxolitinib blocked these IFN-beta anti-inflammatory effects, implicating JAK signaling. Analysis of culture medium zymograms from CF alone, and CF-neutrophils interaction, revealed that MMP2 was mainly originated from CF, while MMP9 could come from neutrophils. LPS and IFN-beta boosted MMP2 secretion by CF. MMP9 activity in CF was low, and LPS or IFN-beta had no significant impact. Pre-treating CF with LPS, IFN-beta, or both before co-culture with neutrophils increased MMP2. Neutrophil co-culture increased MMP9 activity, with IFN-beta pre-treatment reducing MMP9 compared to unstimulated CF.Conclusion: In CF, LPS induces the secretion of IL-8 favoring neutrophils recruitment and these effects were blocked by IFN-. The results highlight that CF-neutrophil interaction appears to influence the extracellular matrix through MMPs activity modulation.
dc.description.funder"Agencia Nacional de Investigacion y Desarrollo de Chile (ANID)" FONDECYT
dc.fuente.origenWOS
dc.identifier.doi10.3389/fcell.2023.1122408
dc.identifier.issn2296-634X
dc.identifier.urihttps://doi.org/10.3389/fcell.2023.1122408
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91660
dc.identifier.wosidWOS:001076989100001
dc.language.isoen
dc.revistaFrontiers in cell and developmental biology
dc.rightsacceso restringido
dc.subjectInterleukin-8
dc.subjectneutrophils
dc.subjectcardiac fibroblast
dc.subjectmetalloprotease
dc.subjectTLR4
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleInterferon-β decreases LPS-induced neutrophil recruitment to cardiac fibroblasts
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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