Density-dependent adaptive resistance allows swimming bacteria to colonize an antibiotic gradient

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dc.contributor.authorHol, Felix J. H.
dc.contributor.authorHubert, Bert
dc.contributor.authorDekker, Cees
dc.contributor.authorKeymer, Juan E.
dc.date.accessioned2025-01-23T21:32:31Z
dc.date.available2025-01-23T21:32:31Z
dc.date.issued2016
dc.description.abstractDuring antibiotic treatment, antibiotic concentration gradients develop. Little is know regarding the effects of antibiotic gradients on populations of nonresistant bacteria. Using a microfluidic device, we show that high-density motile Escherichia coli populations composed of nonresistant bacteria can, unexpectedly, colonize environments where a lethal concentration of the antibiotic kanamycin is present. Colonizing bacteria establish an adaptively resistant population, which remains viable for over 24 h while exposed to the antibiotic. Quantitative analysis of multiple colonization events shows that collectively swimming bacteria need to exceed a critical population density in order to successfully colonize the antibiotic landscape. After colonization, bacteria are not dormant but show both growth and swimming motility under antibiotic stress. Our results highlight the importance of motility and population density in facilitating adaptive resistance, and indicate that adaptive resistance may be a first step to the emergence of genetically encoded resistance in landscapes of antibiotic gradients.
dc.fuente.origenWOS
dc.identifier.doi10.1038/ismej.2015.107
dc.identifier.eissn1751-7370
dc.identifier.issn1751-7362
dc.identifier.urihttps://doi.org/10.1038/ismej.2015.107
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101494
dc.identifier.wosidWOS:000366671300005
dc.issue.numero1
dc.language.isoen
dc.pagina.final38
dc.pagina.inicio30
dc.revistaIsme journal
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDensity-dependent adaptive resistance allows swimming bacteria to colonize an antibiotic gradient
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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