Impact of the Potential Antitumor Agent 2-(4-Hydroxyphenyl) Amino-1,4-Naphthoquinone (Q7) on Vasomotion Is Mediated by the Vascular Endothelium, But Not Vascular Smooth Muscle Cell Metabolism

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dc.contributor.authorPalacios, Javier
dc.contributor.authorBenites, Julio
dc.contributor.authorOwen, Gareth, I
dc.contributor.authorMorales, Pablo
dc.contributor.authorChiong, Mario
dc.contributor.authorNwokocha, Chukwuemeka R.
dc.contributor.authorParedes, Adrian
dc.contributor.authorCifuentes, Fredi
dc.date.accessioned2025-01-20T22:21:05Z
dc.date.available2025-01-20T22:21:05Z
dc.date.issued2021
dc.description.abstractVasomotion is defined as rhythmic oscillations in arterial diameter that regulate the blood flow and blood pressure. Because antitumor treatment may impair vascular functions and increase the blood pressure, we sought to evaluate whether a new naphthoquinone derivative, postulated as an antitumor agent, manifests adverse effects on vascular function. In this article, we evaluated the toxicity of 2-(4-hydroxyphenyl) amino-1,4-naphthoquinone (Q7) and its effects on vascular vasomotion in 3 models of vascular structure: endothelial cells, aortic ring, and smooth muscle cells. Although showing nontoxic effects, Q7 inhibited the formation of capillary-like structures of the EA.hy926 endothelial cell line grown on Matrigel. In exvivo experiments with aortic rings precontracted with phenylephrine (PE, 10(-6) M), Q7 (10(-5) M) significantly (P < 0.05) reduced vascular rhythmic contractions induced by the acetylcholine (ACh; 10(-7)-10(-5) M), whereas sodium nitroprusside (a nitric oxide donor; 10(-8) M) recovered the vasomotion. Furthermore, Q7 (10(-5) M) did not decrease KCl-induced vascular rhythmic contractions in the aortic rings precontracted with BaCl2 (a nonselective K+ channel blocker; 10(-3) M). Vascular smooth muscle cells (A7r5) preincubated with Q7 (10(-5) M) for 3 hours also demonstrated a reduced glucose uptake. However, the Adenosine Triphosphate content was unaffected, suggesting that the rapid reduction in vasomotion observed in vascular reactivity experiments did not involve cellular metabolism but may be due to faster mechanisms involving endothelial nitric oxide and K+ channels leading to oscillations in intracellular Ca2+. In summary, the naphthoquinone derivative Q7 presents low cytotoxicity yet may alter the endothelial cell response and vasomotion in the absence of changes in smooth muscle cell metabolism.
dc.fuente.origenWOS
dc.identifier.doi10.1097/FJC.0000000000000940
dc.identifier.eissn1533-4023
dc.identifier.issn0160-2446
dc.identifier.urihttps://doi.org/10.1097/FJC.0000000000000940
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94641
dc.identifier.wosidWOS:000656644700016
dc.issue.numero2
dc.language.isoen
dc.pagina.final252
dc.pagina.inicio245
dc.revistaJournal of cardiovascular pharmacology
dc.rightsacceso restringido
dc.subjectnaphthoquinone
dc.subjectvasomotion
dc.subjectendothelium
dc.subjectnitric oxide
dc.subjectATP
dc.subjectaorta
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleImpact of the Potential Antitumor Agent 2-(4-Hydroxyphenyl) Amino-1,4-Naphthoquinone (Q7) on Vasomotion Is Mediated by the Vascular Endothelium, But Not Vascular Smooth Muscle Cell Metabolism
dc.typeartículo
dc.volumen77
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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