The expression of adhesion molecules in cigarette smoke-induced airways obstruction

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dc.contributor.authorGonzalez, S
dc.contributor.authorHards, J
dc.contributor.authorvanEeden, S
dc.contributor.authorHogg, JC
dc.date.accessioned2025-01-21T01:33:45Z
dc.date.available2025-01-21T01:33:45Z
dc.date.issued1996
dc.description.abstractCigarette smoking produces peripheral airway inflammation in all smokers, and chronic airways obstruction in approximately 20% of heavy smokers, The present study was designed to test the hypothesis that airways obstruction is related to changes in the expression of adhesion molecules involved in the recruitment of cells to sites of inflammation in the lung.
dc.description.abstractFreshly resected lungs from heavy smokers with airways obstruction (n=10) and from heavy smokers with normal lung function (n=10) were collected in the operating room, inflated with optimal cutting temperature (OCT) medium and frozen over liquid nitrogen, Six micrometre thick cryostat sections cut from random samples of this tissue were stained, using immunohistochemistry, with monoclonal antibodies to the adhesion molecules on leucocytes: L-selectin, very late activation antigen-4 (VLA-4), CD11a/CD18, CD11b/CD18, CD11c/CD18; and on endothelial and epithelial surfaces: E-selectin, P-selectin, vascular cell adhesion molecule (VCAM-1), intercellular adhesion molecule (ICAM)-1 and ICAM-2 using the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. The slides were coded and the expression of each molecule scored by three observers using a semiquantitative grading system, Two inducible adhesion molecules, E-selectin on endothelium and CD11b on leucocytes, were also evaluated using quantitative morphometric analysis.
dc.description.abstractThe results showed a distribution of adhesion molecules that was consistent with the inflammatory response in the airways and parenchyma of all subjects hut failed to show any differences between those with or without airways obstruction.
dc.description.abstractWe conclude that development of airways obstruction in heavy smokers cannot be explained by differences in the expression of adhesion molecules known to be involved in the control of cell traffic in the lung.
dc.fuente.origenWOS
dc.identifier.eissn1399-3003
dc.identifier.issn0903-1936
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97466
dc.identifier.wosidWOS:A1996VL73700006
dc.issue.numero10
dc.language.isoen
dc.pagina.final2001
dc.pagina.inicio1995
dc.revistaEuropean respiratory journal
dc.rightsacceso restringido
dc.subjectadhesion molecules
dc.subjectairways obstruction
dc.subjectlung inflammation
dc.subjectsmoking
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe expression of adhesion molecules in cigarette smoke-induced airways obstruction
dc.typeartículo
dc.volumen9
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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