CHARACTERIZATION OF PEROXISOMES IN CHINESE-HAMSTER OVARY CELLS IN CULTURE

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dc.contributor.authorSANTOS, MJ
dc.contributor.authorGARRIDO, J
dc.contributor.authorOLIVER, C
dc.contributor.authorROBBINS, AR
dc.contributor.authorLEIGHTON, F
dc.date.accessioned2025-01-23T19:26:59Z
dc.date.available2025-01-23T19:26:59Z
dc.date.issued1985
dc.description.abstractIn order to explore the potential value of Chinese hamster overay (CHO) cells for the isolation of peroxisomal mutants defective in the peroxisomal fatty acid oxidation system, some characteristics of their peroxisomes were studied. Catalase was detected biochemically and histochemically in peroxisome-like particles in cells or in subcellular fractions prepared by differential centrifugation or isopyknic equilibrium in Percoll or Metrizamide with catalase in the high density fraction of the isopyknic equilibrium gradients. By oxidation system, exhibited an unusually high specific activity, 2.46 .+-. 1.09 mU/mg protein, in CHO cell homogenates, a value comparable to that of rat liver. This enzyme copurifies with catalase in the high density fractions of the isopycnic equilibrium gradients. By analogy with other cell types and from the ultrastructural analysis, it is concluded that these enzymes are contained in peroxisomes. These findings support the value of CHO cells for studies of peroxisomal function and organization.
dc.fuente.origenWOS
dc.identifier.eissn1090-2422
dc.identifier.issn0014-4827
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99677
dc.identifier.wosidWOS:A1985AUX5700019
dc.issue.numero1
dc.language.isoen
dc.pagina.final198
dc.pagina.inicio189
dc.revistaExperimental cell research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCHARACTERIZATION OF PEROXISOMES IN CHINESE-HAMSTER OVARY CELLS IN CULTURE
dc.typeartículo
dc.volumen161
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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