Medium-throughput image-based phenotypic siRNA screen to unveil the molecular basis of B cell polarization

Simple item page
dc.contributor.authorObino, Dorian
dc.contributor.authorMaurin, Mathieu
dc.contributor.authorDingli, Florent
dc.contributor.authorLoew, Damarys
dc.contributor.authorLescure, Aurianne
dc.contributor.authorTerriac, Emmanuel
dc.contributor.authorGoudot, Christel
dc.contributor.authorMalbec, Odile
dc.contributor.authorLankar, Danielle
dc.contributor.authorYuseff, Maria-Isabel
dc.contributor.authorLennon-Dumenil, Ana-Maria
dc.contributor.authorMoreau, Helene D.
dc.date.accessioned2025-01-20T20:09:46Z
dc.date.available2025-01-20T20:09:46Z
dc.date.issued2023
dc.description.abstractCell polarity is an essential and highly conserved process governing cell function. Cell polarization is generally triggered by an external signal that induces the relocation of the centrosome, thus defining the polarity axis of the cell. Here, we took advantage of B cells as a model to study cell polarity and perform a medium-throughput siRNA-based imaging screen to identify new molecular regulators of polarization. We first identified candidates based on a quantitative proteomic analysis of proteins differentially associated with the centrosome of resting non-polarized and stimulated polarized B cells. We then targeted 233 candidates in a siRNA screen and identified hits regulating the polarization of the centrosome and/or lysosomes in B cells upon stimulation. Our dataset of proteomics, images, and polarity indexes provides a valuable source of information for a broad community of scientists interested in the molecular mechanisms regulating cell polarity.
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41597-023-02301-0
dc.identifier.eissn2052-4463
dc.identifier.urihttps://doi.org/10.1038/s41597-023-02301-0
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92017
dc.identifier.wosidWOS:001013266000003
dc.issue.numero1
dc.language.isoen
dc.revistaScientific data
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMedium-throughput image-based phenotypic siRNA screen to unveil the molecular basis of B cell polarization
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas