Procedure for radiolabeling gizzerosine and basis for a radioimmunoassay

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dc.contributor.authorTorres, M
dc.contributor.authorManosalva, H
dc.contributor.authorCarrasco, I
dc.contributor.authorDe Ioannes, AE
dc.contributor.authorBecker, MI
dc.date.accessioned2025-01-21T01:31:52Z
dc.date.available2025-01-21T01:31:52Z
dc.date.issued1999
dc.description.abstractA method for the labeling of gizzerosine (GZ), a biogenic amine found in fish meal, is described. The labeling procedure with I-125 using a water-soluble Bolton-Hunter reagent and a mild water-insoluble oxidant (Iodogen) reagent is rapid and reproducible. The I-125-GZ hapten was demonstrated to be immunologically active in a radioimmunoassay developed with polyclonal antibodies to GZ absorbed with a histamine-Sepharose column. The curves were linear in the range of 0.0001 and 0.1 mu g/mL. Samples of fish meal previously extracted of histamine with methanol and submitted to acid hydrolysis were contaminated with known amounts of GZ and submitted to the assay. The fish meal samples contaminated with GZ show a dose-response effect similar to the standard curve, and apparently the other component present in the sample did not interfere with the binding of the antibodies to I-125-GZ. These data indicate the suitability of the radioimmunoassay to determine specifically GZ in fish meal.
dc.fuente.origenWOS
dc.identifier.eissn1520-5118
dc.identifier.issn0021-8561
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97158
dc.identifier.wosidWOS:000083242100047
dc.issue.numero10
dc.language.isoen
dc.pagina.final4236
dc.pagina.inicio4231
dc.revistaJournal of agricultural and food chemistry
dc.rightsacceso restringido
dc.subjectgizzerosine labeling
dc.subjectpolyclonal antibodies
dc.subjectRIA
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleProcedure for radiolabeling gizzerosine and basis for a radioimmunoassay
dc.typeartículo
dc.volumen47
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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