Glycated Albumin Triggers an Inflammatory Response in the Human Airway Epithelium and Causes an Increase in Ciliary Beat Frequency

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dc.contributor.authorAitken, Moira L.
dc.contributor.authorSomayaji, Ranjani
dc.contributor.authorHinds, Thomas R.
dc.contributor.authorPier, Maricela
dc.contributor.authorDroguett, Karla
dc.contributor.authorRios, Mariana
dc.contributor.authorSkerrett, Shawn J.
dc.contributor.authorVillalon, Manuel
dc.date.accessioned2025-01-20T23:50:57Z
dc.date.available2025-01-20T23:50:57Z
dc.date.issued2021
dc.description.abstractThe role of inflammation in airway epithelial cells and its regulation are important in several respiratory diseases. When disease is present, the barrier between the pulmonary circulation and the airway epithelium is damaged, allowing serum proteins to enter the airways. We identified that human glycated albumin (GA) is a molecule in human serum that triggers an inflammatory response in human airway epithelial cultures. We observed that single-donor human serum induced IL-8 secretion from primary human airway epithelial cells and from a cystic fibrosis airway cell line (CF1-16) in a dose-dependent manner. IL-8 secretion from airway epithelial cells was time dependent and rapidly increased in the first 4 h of incubation. Stimulation with GA promoted epithelial cells to secrete IL-8, and this increase was blocked by the anti-GA antibody. The IL-8 secretion induced by serum GA was 10-50-fold more potent than TNF alpha or LPS stimulation. GA also has a functional effect on airway epithelial cells in vitro, increasing ciliary beat frequency. Our results demonstrate that the serum molecule GA is pro-inflammatory and triggers host defense responses including increases in IL-8 secretion and ciliary beat frequency in the human airway epithelium. Although the binding site of GA has not yet been described, it is possible that GA could bind to the receptor for advanced glycated end products (RAGE), known to be expressed in the airway epithelium; however, further experiments are needed to identify the mechanism involved. We highlight a possible role for GA in airway inflammation.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fphys.2021.653177
dc.identifier.eissn1664-042X
dc.identifier.urihttps://doi.org/10.3389/fphys.2021.653177
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94753
dc.identifier.wosidWOS:000647668200001
dc.language.isoen
dc.revistaFrontiers in physiology
dc.rightsacceso restringido
dc.subjectairways
dc.subjectairway disease
dc.subjectglycated albumin
dc.subjectinflammation
dc.subjectcytokine
dc.subjecthuman
dc.subjectciliary beat frequency
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGlycated Albumin Triggers an Inflammatory Response in the Human Airway Epithelium and Causes an Increase in Ciliary Beat Frequency
dc.typeartículo
dc.volumen12
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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