SPECIFICITY OF THE AMIDASE AND KININOGENASE METHODS FOR THE DETERMINATION OF RAT URINARY KALLIKREIN

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dc.contributor.authorBERTHOUD, VM
dc.contributor.authorCORTHORN, JL
dc.date.accessioned2025-01-23T19:25:31Z
dc.date.available2025-01-23T19:25:31Z
dc.date.issued1987
dc.description.abstractThe specificity of the amidase and kininogenase methods for determining rat urinary kallikrein was studied. Male and female rat urine was employed. Esterase A1, A2 and kallikrein were separated by DEAE-Sephadex A-50 chromatography. Esterase A1 showed no amidase activity towards the substrate H-D-Val-Leu-Arg-p-nitroanilide. In contrast, esterase A2 and kallikrein attacked the substrate, and the activity of kallikrein was especially inhibited by aprotinin, while esterase A2 was more sensitive to soybean trypsin inhibitor. Esterase A1 did not show kininogenase activity, whereas esterase A2 showed this activity, but only towards the dog plasma substrate. Kallikrein possessed kininogenase activity towards both dog and rat plasma kininogen. We believe that the most specific method for measuring rat urinary kallikrein activity is the kininogenase method using partially purified rat plasma kininogen.
dc.fuente.origenWOS
dc.identifier.issn0340-076X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99493
dc.identifier.wosidWOS:A1987K339400032
dc.issue.numero9
dc.language.isoen
dc.pagina.final600
dc.pagina.inicio595
dc.revistaJournal of clinical chemistry and clinical biochemistry
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSPECIFICITY OF THE AMIDASE AND KININOGENASE METHODS FOR THE DETERMINATION OF RAT URINARY KALLIKREIN
dc.typeartículo
dc.volumen25
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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