PC12 and neuro 2a cells have different susceptibilities to acetylcholinesterase-amyloid complexes, amyloid<sub>25-35</sub> fragment, glutamate, and hydrogen peroxide

Simple item page
dc.contributor.authorCalderón, FH
dc.contributor.authorBonnefont, A
dc.contributor.authorMuñoz, FJ
dc.contributor.authorFernández, V
dc.contributor.authorVidela, LA
dc.contributor.authorInestrosa, NC
dc.date.accessioned2025-01-21T01:32:01Z
dc.date.available2025-01-21T01:32:01Z
dc.date.issued1999
dc.description.abstractThis work addresses the differential effects of several oxidative insults on two neuronal cell lines, PC12 and Neuro 2a cells, extensively used as neuronal models in vitro, We measured cellular damage using the cytotoxic assays for MTT reduction and LDH release and found that acetylcholinesterase (AChE)-amyloid-beta-peptide (AP) complexes, A beta(25-35) fragment, glutamate and H2O2 were over 200-fold more toxic to PC12 than to Neuro 2a cells, 17 alpha and 17 beta estradiol were able to protect both cell types from damage caused by H2O2 or glutamate, By contrast, other insults not related to oxidative stress, such as those caused by the nonionic detergent Triton X-100 and serum deprivation, induced a similar level of damage in both PC12 and Neuro 2a cells, Considering that the AP peptide, H2O2 and glutamate are cellular insults that cause an increase In reactive oxygen species (ROS), the intracellular levels of the antioxidant compound, glutathione were verified, Neuro 2a cells were found to have 4- to 5-fold more glutathione than PC12 cells, Our results suggest that Neuro 2a cells are less susceptible to exposure to AChE-A beta complexes, A beta(25-35) fragment, glutamate and H2O2 than PC12 cells, due to higher intracellular levels of antioxidant defense factors. (C) 1999 Wiley-Liss, Inc.
dc.fuente.origenWOS
dc.identifier.eissn1097-4547
dc.identifier.issn0360-4012
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97188
dc.identifier.wosidWOS:000080526700008
dc.issue.numero6
dc.language.isoen
dc.pagina.final631
dc.pagina.inicio620
dc.revistaJournal of neuroscience research
dc.rightsacceso restringido
dc.subjectoxidative stress
dc.subjectPC12
dc.subjectneuro 2a
dc.subjectglutathione
dc.subjectA beta amyloid
dc.subjectacetylcholinesterase
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePC12 and neuro 2a cells have different susceptibilities to acetylcholinesterase-amyloid complexes, amyloid<sub>25-35</sub> fragment, glutamate, and hydrogen peroxide
dc.typeartículo
dc.volumen56
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas