SPERM OOCYTE INTERACTION - STUDIES ON THE KINETICS OF ZONA-PELLUCIDA BINDING AND ACROSOME REACTION OF HUMAN SPERMATOZOA

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dc.contributor.authorMORALES, P
dc.contributor.authorVIGIL, P
dc.contributor.authorFRANKEN, DR
dc.contributor.authorKASKAR, K
dc.contributor.authorCOETZEE, K
dc.contributor.authorKRUGER, TF
dc.date.accessioned2025-01-21T01:35:32Z
dc.date.available2025-01-21T01:35:32Z
dc.date.issued1994
dc.description.abstractSuccessful sperm-oocyte interaction depends, among other things, on sperm capacitation, which is defined by acrosomal and motility alterations. In the study described here the authors evaluated different aspects of this gamete interaction in humans. Specifically, the authors studied (1) the relationship between the number of spermatozoa bound to the zona pellucida and sperm concentration and incubation period, (2) the capacitation status and kinetics of acrosome reaction among the zona-bound spermatozoa, and (3) the effect of human follicular fluid on the zona-binding potential and acrosome status of spermatozoa from different men. The results indicated a concentration of 10(7) cells ml-1 after 15 min of coincubation to be the optimum for zona binding. The number of sperm bound after 0, 3 and 5 h of incubation was the same. In addition, spermatozoa incubated for 3 or 5 h under-went the acrosome reaction (range 9-43%) on the zona surface within 15 min of binding. The maximum percentage of acrosome-reacted spermatozoa was reached after 60 min of binding. Follicular fluid affected the sperm populations selectively, since it did not influence zona binding capacity in all cases. The data enhances the authors' understanding of critical events occurring before fertilization.
dc.fuente.origenWOS
dc.identifier.eissn1439-0272
dc.identifier.issn0303-4569
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97693
dc.identifier.wosidWOS:A1994NN74800003
dc.issue.numero3
dc.language.isoen
dc.pagina.final137
dc.pagina.inicio131
dc.revistaAndrologia
dc.rightsacceso restringido
dc.subjectSPERMATOZOA
dc.subjectACROSOME REACTION
dc.subjectZONA-PELLUCIDA
dc.subjectFOLLICULAR FLUID
dc.subjectHUMAN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSPERM OOCYTE INTERACTION - STUDIES ON THE KINETICS OF ZONA-PELLUCIDA BINDING AND ACROSOME REACTION OF HUMAN SPERMATOZOA
dc.typeartículo
dc.volumen26
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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