Characterization of Endogenous Levels of Brassinosteroids and Related Genes in Grapevines

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dc.contributor.authorParada, Francisca
dc.contributor.authorOklestkova, Jana
dc.contributor.authorArce-Johnson, Patricio
dc.date.accessioned2025-01-20T22:00:54Z
dc.date.available2025-01-20T22:00:54Z
dc.date.issued2022
dc.description.abstractAgronomic breeding practices for grapevines (Vitis vinifera L.) include the application of growth regulators in the field. Brassinosteroids (BRs) are a family of sterol-derived plant hormones that regulate several physiological processes and responses to biotic and abiotic stress. In grapevine berries, the production of biologically active BRs, castasterone and 6-deoxocastasterone, has been reported. In this work, key BR genes were identified, and their expression profiles were determined in grapevine. Bioinformatic homology analyses of the Arabidopsis genome found 14 genes associated with biosynthetic, perception and signaling pathways, suggesting a partial conservation of these pathways between the two species. The tissue- and development-specific expression profiles of these genes were determined by qRT-PCR in nine different grapevine tissues. Using UHPLC-MS/MS, 10 different BR compounds were pinpointed and quantified in 20 different tissues, each presenting specific accumulation patterns. Although, in general, the expression profile of the biosynthesis pathway genes of BRs did not directly correlate with the accumulation of metabolites, this could reflect the complexity of the BR biosynthesis pathway and its regulation. The development of this work thus generates a contribution to our knowledge about the presence, and diversity of BRs in grapevines.
dc.fuente.origenWOS
dc.identifier.doi10.3390/ijms23031827
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://doi.org/10.3390/ijms23031827
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93762
dc.identifier.wosidWOS:000923818900001
dc.issue.numero3
dc.language.isoen
dc.revistaInternational journal of molecular sciences
dc.rightsacceso restringido
dc.subjectbrassinosteroids
dc.subjectgrapevine
dc.subjectdevelopment
dc.subjectUHPLC-MS/MS
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleCharacterization of Endogenous Levels of Brassinosteroids and Related Genes in Grapevines
dc.typeartículo
dc.volumen23
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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