CHARACTERIZATION OF THE AMYLOLYTIC ACTIVITY OF ARAUCARIA-ARAUCANA (MOL) KOCH GERMINATING-SEEDS

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dc.contributor.authorREINERO, A
dc.contributor.authorBALBOA, O
dc.contributor.authorCARDEMIL, L
dc.date.accessioned2025-01-23T19:43:54Z
dc.date.available2025-01-23T19:43:54Z
dc.date.issued1983
dc.description.abstractStarch is the main reserve in A. araucana (Mol.) Koch seeds. To understand the regulation of starch mobilization during seed germination, amylase activity was characterized and its levels were measured for the first 90 h after the start of seed imbibition. Amylase is present in the embryo and megagametophyte when seeds are quiescent. The enzyme activity of the embryo has 2 peaks during the 90 h, while amylase activity of the megagametophyte remains low and unchanged during this time. Analysis of the products released by the enzyme and thermostability experiments identify the amylase as .alpha.-amylase. Differential centrifugation of embryo homogenates under isoosmotic conditions shows that the enzyme is soluble. Quantification of the endogenous GA of the embryo shows a time correlation between the rise in amylase activity and the rise in specific GA levels during the 40 h after the start of imbibition. The growth retardant AMO-1618 [N,N,N-2-tetramethyl-5-(1-methylethyl)-4-[(1-piperidinylcarbonyl)oxy]-benzenaminium chloride] lowers enzyme activity to 37.3% of the control at this time. Activity is almost completely restored when the GA from embryonic tissue are added in physiological doses to the imbibition medium containing AMO-1618.
dc.fuente.origenWOS
dc.identifier.eissn1471-9053
dc.identifier.issn0032-0781
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99866
dc.identifier.wosidWOS:A1983QS52100018
dc.issue.numero3
dc.language.isoen
dc.pagina.final465
dc.pagina.inicio457
dc.revistaPlant and cell physiology
dc.rightsacceso restringido
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleCHARACTERIZATION OF THE AMYLOLYTIC ACTIVITY OF ARAUCARIA-ARAUCANA (MOL) KOCH GERMINATING-SEEDS
dc.typeartículo
dc.volumen24
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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