Bile Acids Induce Alterations in Mitochondrial Function in Skeletal Muscle Fibers

Simple item page
dc.contributor.authorAbrigo, Johanna
dc.contributor.authorOlguin, Hugo
dc.contributor.authorGutierrez, Danae
dc.contributor.authorTacchi, Franco
dc.contributor.authorArrese, Marco
dc.contributor.authorCabrera, Daniel
dc.contributor.authorValero-Breton, Mayalen
dc.contributor.authorElorza, Alvaro A.
dc.contributor.authorSimon, Felipe
dc.contributor.authorCabello-Verrugio, Claudio
dc.date.accessioned2025-01-20T21:02:30Z
dc.date.available2025-01-20T21:02:30Z
dc.date.issued2022
dc.description.abstractCholestatic chronic liver disease is characterized by developing sarcopenia and elevated serum levels of bile acids. Sarcopenia is a skeletal muscle disorder with the hallmarks of muscle weakness, muscle mass loss, and muscle strength decline. Our previous report demonstrated that deoxycholic acid (DCA) and cholic acid (CA), through the membrane receptor TGR5, induce a sarcopenia-like phenotype in myotubes and muscle fibers. The present study aimed to evaluate the impact of DCA and CA on mitochondrial mass and function in muscle fibers and the role of the TGR5 receptor. To this end, muscle fibers obtained from wild-type and TGR5(-/-) mice were incubated with DCA and CA. Our results indicated that DCA and CA decreased mitochondrial mass, DNA, and potential in a TGR5-dependent fashion. Furthermore, with TGR5 participation, DCA and CA also reduced the oxygen consumption rate and complexes I and II from the mitochondrial electron transport chain. In addition, DCA and CA generated more mitochondrial reactive oxygen species than the control, which were abolished in TGR5(-/-) mice muscle fibers. Our results indicate that DCA and CA induce mitochondrial dysfunction in muscle fibers through a TGR5-dependent mechanism.
dc.fuente.origenWOS
dc.identifier.doi10.3390/antiox11091706
dc.identifier.eissn2076-3921
dc.identifier.urihttps://doi.org/10.3390/antiox11091706
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93045
dc.identifier.wosidWOS:000858090400001
dc.issue.numero9
dc.language.isoen
dc.revistaAntioxidants
dc.rightsacceso restringido
dc.subjectbile acids
dc.subjectmitochondria
dc.subjectsarcopenia
dc.subjectmuscle wasting
dc.subjectTGR5 receptor
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBile Acids Induce Alterations in Mitochondrial Function in Skeletal Muscle Fibers
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas