Crosslinking of amyloid-β peptide to brain acetylcholinesterase

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dc.contributor.authorOpazo, C
dc.contributor.authorInestrosa, NC
dc.date.accessioned2025-01-21T01:32:53Z
dc.date.available2025-01-21T01:32:53Z
dc.date.issued1998
dc.description.abstractAcetylcholinesterase (AChE) is the enzyme responsible for the hydrolysis of the neurotransmitter acetylcholine in the central nervous system. Recently, we have found that AChE promotes the assembly of amyloid-beta peptides (A beta) into Alzheimer fibrils. The action of AChE on the state of aggregation of the A beta peptide supposes a near neighbor relationship between these two molecules. In the present work, we have studied A beta-AChE interactions using the crosslinker reagent disuccinimidyl suberate (DSS), in the presence of [I-125]-A beta peptide The A beta-AChE complexes formed by crosslinking were then analyzed by SDS-PAGE and autoradiography. We observed the formation of [I-125] A beta-labeled complexes of 70, 160, 250, and 300 kDa corresponding to monomers, dimers, tetramers, and oligomers of AChE, respectively crosslinked with the A beta peptide. Our results suggest that AChE and the A beta peptide may be involved in physiologically relevant interactions, related to the pathogenesis of Alzheimer disease (AD).
dc.fuente.origenWOS
dc.identifier.issn1044-7393
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97338
dc.identifier.wosidWOS:000071935100004
dc.issue.numero1
dc.language.isoen
dc.pagina.final49
dc.pagina.inicio39
dc.revistaMolecular and chemical neuropathology
dc.rightsacceso restringido
dc.subjectAlzheimer disease
dc.subjectamyloid-beta
dc.subjectacetylcholinesterase
dc.subjectcrosslinking
dc.subjectA beta-AChE complex
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCrosslinking of amyloid-β peptide to brain acetylcholinesterase
dc.typeartículo
dc.volumen33
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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