Viral shedding dynamics reveals sputum as a reliable and cost-saving specimen for SARS-CoV-2 diagnosis within the first 10 days since symptom onset: A prospective cohort study

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dc.catalogadorpau
dc.contributor.authorLevican, Jorge
dc.contributor.authorAlmonacid, Leonardo I.
dc.contributor.authorValenzuela, Gonzalo
dc.contributor.authorGarcía-Salum, Tamara
dc.contributor.authorRojas, Luis
dc.contributor.authorSerrano, Eileen
dc.contributor.authorPardo-Roa, Catalina
dc.contributor.authorSalinas, Erick
dc.contributor.authorAvendaño, María José
dc.contributor.authorPerazzo, Fabiola
dc.contributor.authorDíaz, Luis Antonio
dc.contributor.authorValderrama, Sebastián
dc.contributor.authorOrtega, Marcos
dc.contributor.authorToro, Adriana
dc.contributor.authorMontecinos, Viviana
dc.contributor.authorRiquelme, Arnoldo
dc.contributor.authorMedina, Rafael A.
dc.date.accessioned2025-07-03T14:31:53Z
dc.date.available2025-07-03T14:31:53Z
dc.date.issued2020
dc.description.abstractCoronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome virus (SARS-CoV-2) is challenging global public health, due to an increasing demand for testing and the shortage of diagnostic supplies. Nasopharyngeal swab (NPS) is considered the optimal sample for SARS-CoV2 diagnosis and sputum (SPT) has been proposed as an economic alternative. However, the temporal concordance of diagnosis in NPS and SPT has not been addressed. Methods: Through a longitudinal study we compared the shedding dynamics of SARS-CoV-2 RNA evaluated by RT-qPCR in serially collected SPT and NPS obtained from 82 ambulatory and hospitalized patients during acute infection and convalescence. The concordance during the follow-up and cost analysis between both collected specimens was evaluated. Findings: We analyzed 379 samples, 177 NPS and 202 SPT. The highest proportion of positive samples was detected within the first 15 days after the symptoms onset. The median time of positivity was higher for NPS (median= 25 days) than SPT (median= 21 days). There was no significant difference in the median RT-qPCR CT values between both sample types. The temporal categorization of matched-paired samples indicated substantial correlation (r=0·6023) and substantial agreement (87·23%) during the first ten days since symptoms onset (kappa = 0·697). A cost analysis demonstrated a significant saving when the SPT specimen was used. Interpretation: Sputum is a feasible and cost-saving alternative to NPS, providing an equivalent value for the detection and follow-up of SARS-CoV-2 RNA.
dc.fechaingreso.objetodigital2025-07-03
dc.fuente.origenORCID
dc.identifier.doi10.1101/2020.08.30.20183889
dc.identifier.urihttps://doi.org/10.1101/2020.08.30.20183889
dc.identifier.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-85098614331&partnerID=MN8TOARS
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/104835
dc.information.autorucFacultad de Ciencias Biológicas; Almonacid, Leonardo I.; S/I; 250112
dc.information.autorucEscuela de Medicina; García-Salum, Tamara; S/I; 121228
dc.information.autorucFacultad de Ciencias Biológicas; Serrano, Eileen; S/I; 1086419
dc.information.autorucFacultad de Ciencias Biológicas; Pardo-Roa, Catalina; 0000-0002-5409-225X; 225251
dc.information.autorucEscuela de Medicina; Salinas, Erick; S/I; 1101975
dc.information.autorucFacultad de Ciencias Biológicas; Avendaño, María José; S/I; 1086420
dc.information.autorucEscuela de Medicina; Perazzo, Fabiola; S/I; 234449
dc.information.autorucEscuela de Medicina; Díaz, Luis Antonio; 0000-0002-8540-4930; 179253
dc.information.autorucEscuela de Medicina; Valderrama, Sebastián; S/I; 120766
dc.information.autorucEscuela de Medicina; Ortega, Marcos; S/I; 119643
dc.information.autorucEscuela de Medicina; Montecinos, Viviana; S/I; 111594
dc.information.autorucEscuela de Medicina; Riquelme, Arnoldo; 0000-0002-8259-8960; 3538
dc.information.autorucEscuela de Medicina; Medina, Rafael A.; 0000-0002-5013-6884; 1000649
dc.language.isoen
dc.nota.accesocontenido completo
dc.rightsacceso abierto
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleViral shedding dynamics reveals sputum as a reliable and cost-saving specimen for SARS-CoV-2 diagnosis within the first 10 days since symptom onset: A prospective cohort study
dc.typepre-print
sipa.codpersvinculados250112
sipa.codpersvinculados121228
sipa.codpersvinculados1086419
sipa.codpersvinculados225251
sipa.codpersvinculados1101975
sipa.codpersvinculados1086420
sipa.codpersvinculados234449
sipa.codpersvinculados179253
sipa.codpersvinculados120766
sipa.codpersvinculados119643
sipa.codpersvinculados111594
sipa.codpersvinculados3538
sipa.codpersvinculados1000649
sipa.trazabilidadORCID;2025-06-23
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