Renal cyclooxygenase-2 - Evidence for recruitment of thick ascending limb of Henle cells in microdissected nephron segments

Simple item page
dc.contributor.authorLeon, CG
dc.contributor.authorMarchetti, J
dc.contributor.authorVio, CP
dc.date.accessioned2025-01-21T01:30:45Z
dc.date.available2025-01-21T01:30:45Z
dc.date.issued2001
dc.description.abstractProstaglandins participate in the regulation of sodium and water renal excretion. They are synthesized by cyclooxygenases (COX): the constitutive isoform and the enzyme regulated by physiological stimuli (COX-2). Our previous immunohistochemical studies have demonstrated the presence of COX-2 in a subset of thick ascending limb (TAL) of Henle cells and its induction during the postnatal period and after adrenalectomy. Previous results suggested that this induction phenomenon proceeds by recruitment of TAL cells from the cortex to the outer medulla. The present work aimed to specifically address these preliminary observations by using immunohistochemical techniques in single microdissected nephron segments. Normal adult rats, adrenalectomized rats, adrenalectomized rats on dexamethasone and 5, 10, and 15 days postnatal age were used (Sprague-Dawley rats, n=5 each group). Glomeruli and different segments of nephron were microdissected from collagenase-treated kidney tissue. Tubules were immunostained with specific antibodies against COX-2. We confirmed that COX-2 was localized exclusively in TAL segments; it was induced after adrenalectomy and during postnatal age, peaking at 15 days after birth. We provided morphological evidence that the induction of COX-2 along TAL proceeded in a defined pattern by recruitment of cells from the cortical portion close to the glomeruli toward the outer medulla. No COX-2 was observed in the post-macula densa portion of the segments. Our results provide the anatomical basis for the contribution of COX-2 in physiological mechanisms such as renin secretion, tubuloglomerular feedback, and the interaction with neuronal NO synthase at the juxtaglomerular apparatus.
dc.fuente.origenWOS
dc.identifier.eissn1524-4563
dc.identifier.issn0194-911X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96877
dc.identifier.wosidWOS:000171308400020
dc.issue.numero3
dc.language.isoen
dc.pagina.final634
dc.pagina.inicio630
dc.revistaHypertension
dc.rightsacceso restringido
dc.subjectcyclooxygenase
dc.subjectkidney
dc.subjectrat
dc.subjectimmuno histochemistry
dc.subjectglucocorticoids
dc.subjectprostaglandins
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRenal cyclooxygenase-2 - Evidence for recruitment of thick ascending limb of Henle cells in microdissected nephron segments
dc.typeartículo
dc.volumen38
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas