CELL-GROWTH AND NA-K-CL COTRANSPORT RESPONSES OF VASCULAR SMOOTH-MUSCLE CELLS OF MILAN RATS

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dc.contributor.authorCANESSA, M
dc.contributor.authorSALAZAR, G
dc.contributor.authorWERNER, E
dc.contributor.authorVALLEGA, G
dc.contributor.authorGONZALEZ, A
dc.date.accessioned2024-01-10T13:46:36Z
dc.date.available2024-01-10T13:46:36Z
dc.date.issued1994
dc.description.abstractThe present study examines the role of serum growth factors in the proliferative response and Na-K-Cl cotransport activity of vascular smooth muscle cells from Milan normotensive (MNS) and hypertensive (MHS) rats. Cells from thoracic aorta of both strains were cultured in 10% serum medium and made quiescent by 72 hours in 0.3% serum medium. MHS cells grown with 10% serum had a shorter population doubling time than MNS cells between passages 8 and 12 (13.8+/-1.7 versus 20.1+/-1.6 hours, P<.0l, n=4). MHS cells also exhibited a higher response of thymidine incorporation into nucleic acid to serum, epidermal, and platelet-derived growth factor BB. In MHS cells epidermal (100 ng/mL) and platelet (50 ng/mL) growth factors increased thymidine incorporation 2- and 10-fold, respectively. In MNS cells epidermal factor did not induce a significant response, and that of platelet factor was twofold lower than in MHS cells. Binding curves revealed a higher number of receptors for platelet than epidermal growth factor in both strains and a similar number of both receptors in MHS and MNS cells. Quantitative immunoblots of these receptor proteins confirmed the observation that the greater proliferation of MHS cells could not be related to a higher number of growth factor receptors. Cotransport activity (bumetanide-sensitive Rb-86 influx in nanomoles per milligram protein per 5 minutes) was found to be significantly higher in MHS cells (16+/-3, n=18) than MNS cells (8+/-3, n=15) at confluence as well as in the log phase of serum-stimulated growth. No differences were found between strains with cells in the quiescent state. However, platelet growth factor (50 ng/mL) markedly stimulated cotransport in quiescent MHS cells, whereas epidermal growth factor was without effect. In conclusion, MHS cells exhibited enhanced serum- and platelet factor-stimulated proliferation rates and cotransport activity compared with MNS cells. These results suggest that platelet factor BB plays an important role in the proliferation of hypertensive vascular cells.
dc.description.funderNHLBI NIH HHS
dc.description.funderNATIONAL HEART, LUNG, AND BLOOD INSTITUTE
dc.fechaingreso.objetodigital2024-05-16
dc.format.extent5 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1161/01.HYP.23.6.1022
dc.identifier.eissn1524-4563
dc.identifier.issn0194-911X
dc.identifier.pubmedidMEDLINE:8206586
dc.identifier.urihttps://doi.org/10.1161/01.HYP.23.6.1022
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79178
dc.identifier.wosidWOS:A1994NR58000043
dc.information.autorucMedicina;Gonzalez L;S/I;52306
dc.issue.numero6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final1026
dc.pagina.inicio1022
dc.publisherLIPPINCOTT WILLIAMS & WILKINS
dc.revistaHYPERTENSION
dc.rightsacceso restringido
dc.subjectMILAN RATS
dc.subjectPDGF BB
dc.subjectSMOOTH MUSCLE
dc.subjectCELL PROLIFERATION
dc.subjectNA-K-CL COTRANSPORT
dc.subjectSPONTANEOUSLY HYPERTENSIVE RATS
dc.subjectFIBROBLASTS
dc.subjectPROTEINS
dc.subjectRECEPTOR
dc.subjectBINDING
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCELL-GROWTH AND NA-K-CL COTRANSPORT RESPONSES OF VASCULAR SMOOTH-MUSCLE CELLS OF MILAN RATS
dc.typeartículo
dc.volumen23
sipa.codpersvinculados52306
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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