PENTOXIFYLLINE INCREASES SPERM PENETRATION INTO ZONA-FREE HAMSTER OOCYTES WITHOUT INCREASING THE ACROSOME REACTION

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dc.contributor.authorMORALES, P
dc.contributor.authorLLANOS, M
dc.contributor.authorYOVICH, JL
dc.contributor.authorCUMMINS, JM
dc.contributor.authorVIGIL, P
dc.date.accessioned2025-01-23T19:21:37Z
dc.date.available2025-01-23T19:21:37Z
dc.date.issued1993
dc.description.abstractSeveral drugs have been used to stimulate human sperm motility, including 3-deoxy-adenosine, caffeine, and pentoxifylline. Pentoxifylline is an inhibitor of the phosphodiesterase and may stimulate sperm motility by increasing the intracellular levels of cAMP. In this study we have evaluated the effect of pentoxifylline in the outcome of the sperm penetration assay into zona-free hamster oocytes. Twenty-seven semen samples, obtained for diagnostic purposes, were used. After the motile sperm were selected by the swim-up technique, the samples were divided into two aliquots. One aliquot was incubated with 1 mg ml-1 of pentoxifylline at 37-degrees-C, 5% CO2 for 30 min. The control aliquot was incubated with culture medium. The samples were then washed and resuspended in fresh, pentoxifylline-free medium, at a sperm concentration of 10 X 10(6) cells ml-1. One hundred microlitres of each sperm suspension was then deposited under oil and 30-40 zona-free hamster oocytes were added. After 6 h of gamete coincubation, the percentage of penetrated oocytes and the number of decondensed sperm heads were evaluated. The percentage of acrosome-reacted sperm was evaluated using the Pisum sativum lectin. The percentage of zona-free hamster oocytes penetrated was increased after pentoxifylline-treatment. The percentage of acrosome reacted sperm and the number of decondensed sperm heads per egg were not different between the control and the pentoxifylline-treated groups. The results suggest that the beneficial effect of pentoxifylline upon the sperm cells is not mediated by stimulation of the acrosome reaction.
dc.fuente.origenWOS
dc.identifier.issn0303-4569
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98817
dc.identifier.wosidWOS:A1993MG44800010
dc.issue.numero6
dc.language.isoen
dc.pagina.final362
dc.pagina.inicio359
dc.revistaAndrologia
dc.rightsacceso restringido
dc.subjectPENTOXIFYLLINE
dc.subjectSPERM PENETRATION ASSAY
dc.subjectACROSOME REACTION
dc.subjectSPERM MOTILITY
dc.subjectHUMAN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePENTOXIFYLLINE INCREASES SPERM PENETRATION INTO ZONA-FREE HAMSTER OOCYTES WITHOUT INCREASING THE ACROSOME REACTION
dc.typeartículo
dc.volumen25
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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