ULTRASTRUCTURAL OBSERVATIONS OF INCORPORATION OF GUINEA-PIG SPERMATOZOA INTO ZONA-FREE HAMSTER OOCYTES
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Date
1977
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Abstract
Zona-free hamster oocytes inseminated in vitro with acrosome-reacted guinea-pig spermatozoa were examined with the EM. Guinea-pig spermatozoa, in the vicinity of the oocytes, consistently lacked the whole acrosome including the equatorial segment region. In cross fertilization, sperm-egg membrane fusion does not differ significantly from that of normal fertilization. It was sometimes possible to observe protrusions of oocyte cytoplasm containing sperm chromatin in the process of dispersion.