Transcriptomics using next generation sequencing technologies

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dc.catalogadoraba
dc.contributor.authorLee Liu, D.
dc.contributor.authorAlmonacid, L. I.
dc.contributor.authorFaunes Quinteros, Fernando Emerson
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorLarraín Correa, Juan Agustín
dc.date.accessioned2025-02-06T18:46:18Z
dc.date.available2025-02-06T18:46:18Z
dc.date.issued2012
dc.description.abstractNext generation sequencing technologies may now be applied to the study of transcriptomics. RNA-Seq or RNA sequencing employs high-throughput sequencing of complementary DNA fragments delivering a transcriptional profile. In this chapter, we aim to provide a starting point for Xenopus researchers planning on starting an RNA-Seq transcriptomics study. We begin by providing a section on template isolation and library preparation. The next section comprises the main bioinformatics procedures that need to be performed for raw data processing, normalization, and differential gene expression. Finally, we have included a section on studying deep sequencing results in Xenopus, which offers general guidance as to what can be done in this model.
dc.fuente.origenSIPA
dc.identifier.doi10.1007/978-1-61779-992-1_18
dc.identifier.eissn1940-6029
dc.identifier.issn1064-3745
dc.identifier.urihttps://doi.org/10.1007/978-1-61779-992-1_18
dc.identifier.urihttps://doi.org/10.1007/978-1-61779-992-1_18
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102167
dc.information.autorucFacultad de Ciencias Biológicas; Faunes Quinteros, Fernando Emerson; S/I; 13517
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.information.autorucFacultad de Ciencias Biológicas; Larraín Correa, Juan Agustín; S/I; 90468
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final317
dc.pagina.inicio293
dc.revistaMethods in molecular biology (Clifton, N.J.)
dc.rightsacceso restringido
dc.subjectXenopus tropicalis
dc.subjectXenopus laevis
dc.subjectRNA-Seq
dc.subjectSmall RNA-Seq
dc.subjectTranscriptomics
dc.subjectTranscriptional profiling
dc.subjectHigh-throughput sequencing
dc.subjectNext generation sequencing
dc.subjectMassively ­parallel sequencing
dc.subjectIllumina
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.titleTranscriptomics using next generation sequencing technologies
dc.typeartículo
dc.volumen917
sipa.codpersvinculados13517
sipa.codpersvinculados82342
sipa.codpersvinculados90468
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