Multiple Labeling of Compartmentalized Cortical Neurons in Microfluidic Chambers

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dc.contributor.authorMoya-Alvarado, Guillermo
dc.contributor.authorAguirre-Soto, Alejandro
dc.contributor.authorBronfman, Francisca C.
dc.date.accessioned2025-01-20T17:08:46Z
dc.date.available2025-01-20T17:08:46Z
dc.date.issued2024
dc.description.abstractNeurons are complex cells with two distinct compartments: the somatodendritic and the axonal domains. Because of their polarized morphology, it is challenging to study the differential cellular and molecular mechanisms that occur in axons and impact the soma and dendrites using conventional in vitro culture systems. Compartmentalized cultures offer a solution by physically and chemically separating the axonal from the somatodendritic domain of neurons. The microfluidic chamber model presented in this work is valuable for studying these mechanisms in primary cortical cultures derived from rat and mouse. In addition, this chamber model is compatible with various microscopy methods, such as phase contrast, and fluorescence imaging of living and fixed cells.
dc.fuente.origenWOS
dc.identifier.doi10.21769/BioProtoc.4911
dc.identifier.eissn2331-8325
dc.identifier.urihttps://doi.org/10.21769/BioProtoc.4911
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90982
dc.identifier.wosidWOS:001165106000004
dc.issue.numero1
dc.language.isoen
dc.revistaBio-protocol
dc.rightsacceso restringido
dc.subjectCompartmentalized cultures
dc.subjectMicrofluidic chambers
dc.subjectCortical neurons
dc.subjectEndocytosis
dc.subjectBDNF
dc.subjectAxons
dc.subjectLong- distance signaling
dc.subjectCholera toxin subunit B
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMultiple Labeling of Compartmentalized Cortical Neurons in Microfluidic Chambers
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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