HIGH POTASSIUM CONCENTRATION IMPROVES THE RATE OF ACROSOME REACTION IN HUMAN-SPERMATOZOA

dc.contributor.authorROBLERO, L
dc.contributor.authorFERNANDEZ, E
dc.contributor.authorGUADARRAMA, A
dc.contributor.authorZEGERSHOCHSCHILD, F
dc.contributor.authorORTIZ, ME
dc.date.accessioned2025-01-23T19:24:53Z
dc.date.available2025-01-23T19:24:53Z
dc.date.issued1988
dc.description.abstractProgressively motile spermatozoa recovered by swim-up method from semen of two fertile men were inoculated for 24 hours in culture media containing either 4.7, 15, or 25 mM of potassium (K). Aliquots of each culture condition were obtained at 0, 1, 5, 10, and 24 hours of incubation for the assessment of progressive motility, percentage of dead spermatozoa, and percentage of acrosome reaction (AR), as measured by triple-stain technique. A total of ten experiments including each K concentration were analyzed. The results of this study showed no effect of K concentration on the percentage of progressively motile spermatozoa, irrespective of the time of incubation. The percentage of live spermatozoa was significantly greater in culture medium containing 25 mM K (P < 0.05). There was a greater percentage of reacted spermatozoa with 25 mM K, as compared with 4.7 and 15 mM (P < 0.05). Furthermore, the time taken to acheive 20% of AR was 2 hours at 25 mM K compared with 10.9 hours at 4.7 mM K.
dc.fuente.origenWOS
dc.identifier.eissn1556-5653
dc.identifier.issn0015-0282
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99413
dc.identifier.wosidWOS:A1988M907300020
dc.issue.numero4
dc.language.isoen
dc.pagina.final679
dc.pagina.inicio676
dc.revistaFertility and sterility
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHIGH POTASSIUM CONCENTRATION IMPROVES THE RATE OF ACROSOME REACTION IN HUMAN-SPERMATOZOA
dc.typeartículo
dc.volumen49
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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