A novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in <i>Bifidobacterium longum</i> subsp <i>longum</i> SC596

Simple item page
dc.contributor.authorGarrido, Daniel
dc.contributor.authorRuiz-Moyano, Santiago
dc.contributor.authorKirmiz, Nina
dc.contributor.authorDavis, Jasmine C.
dc.contributor.authorTotten, Sarah M.
dc.contributor.authorLemay, Danielle G.
dc.contributor.authorUgalde, Juan A.
dc.contributor.authorGerman, J. Bruce
dc.contributor.authorLebrilla, Carlito B.
dc.contributor.authorMills, David A.
dc.date.accessioned2025-01-23T21:28:20Z
dc.date.available2025-01-23T21:28:20Z
dc.date.issued2016
dc.description.abstractThe infant intestinal microbiota is often colonized by two subspecies of Bifidobacterium longum: subsp. infantis (B. infantis) and subsp. longum (B. longum). Competitive growth of B. infantis in the neonate intestine has been linked to the utilization of human milk oligosaccharides (HMO). However, little is known how B. longum consumes HMO. In this study, infant-borne B. longum strains exhibited varying HMO growth phenotypes. While all strains efficiently utilized lacto-N-tetraose, certain strains additionally metabolized fucosylated HMO. B. longum SC596 grew vigorously on HMO, and glycoprofiling revealed a preference for consumption of fucosylated HMO. Transcriptomes of SC596 during early-stage growth on HMO were more similar to growth on fucosyllactose, transiting later to a pattern similar to growth on neutral HMO. B. longum SC596 contains a novel gene cluster devoted to the utilization of fucosylated HMO, including genes for import of fucosylated molecules, fucose metabolism and two alpha-fucosidases. This cluster showed a modular induction during early growth on HMO and fucosyllactose. This work clarifies the genomic and physiological variation of infant-borne B. longum to HMO consumption, which resembles B. infantis. The capability to preferentially consume fucosylated HMO suggests a competitive advantage for these unique B. longum strains in the breast-fed infant gut.
dc.fuente.origenWOS
dc.identifier.doi10.1038/srep35045
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1038/srep35045
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101405
dc.identifier.wosidWOS:000386143200001
dc.language.isoen
dc.revistaScientific reports
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in <i>Bifidobacterium longum</i> subsp <i>longum</i> SC596
dc.typeartículo
dc.volumen6
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas