Comparative effect of platelet-rich plasma, platelet-poor plasma, and fetal bovine serum on the proliferative response of periodontal ligament cell subpopulations

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dc.contributor.authorMartinez, Constanza E.
dc.contributor.authorGomez, Roberto
dc.contributor.authorKalergis, Alexis M.
dc.contributor.authorSmith, Patricio C.
dc.date.accessioned2025-01-23T21:15:36Z
dc.date.available2025-01-23T21:15:36Z
dc.date.issued2019
dc.description.abstractObjectivesCell-based therapies involve the need to expand cell cultures ex vivo for their subsequent implantation in an autologous manner. An important limitation regarding this technology is the use of fetal bovine serum (FBS) that has critical safety limitations. Platelet-derived fractions represent an autologous source of growth factors that may be used for the expansion of these cell cultures. Periodontal ligament (PDL) cells comprise a heterogeneous cell population that may not necessarily respond in a uniform manner to proliferative stimuli. The aim of this study was to evaluate the ability of two platelet-derived fractions, platelet-rich plasma (PRP) and platelet-poor plasma (PPP) and FBS on the proliferative response of different subpopulations of PDL cell cultures.Materials and methodsPDL cells were characterized and then exposed to PRP, PPP, or FBS during 2, 5, or 14days to analyze cell proliferation and clonogenic capability. Cell proliferation was evaluated through immunofluorescence for Ki67 and by tracing carboxyfluorescein diacetate succinimidyl ester (CFSE) dye in combination with mesenchymal stem cell markers using flow cytometry.ResultsBoth PRP and PPP stimulated PDL cell proliferation and their clonogenic ability. We found a significant increase of CD73- and CD90-positive cells after PRP or PPP treatment, compared to FBS. Otherwise, no differences were found regarding the response of CD146-or CD105-positive cells when stimulated with PRP, PPP, or FBS.ConclusionPRP and PPP can stimulate the proliferation and clonogenicity of PDL cell populations including cells positive for CD90 and CD73 markers.Clinical relevanceThese findings may have implications for future therapies aiming to stimulate periodontal regeneration using autologous growth factors.
dc.description.funderFondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) from the Chilean Government and Puente research grant, Pontificia Universidad Catolica de Chile
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00784-018-2637-1
dc.identifier.eissn1436-3771
dc.identifier.issn1432-6981
dc.identifier.urihttps://doi.org/10.1007/s00784-018-2637-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101096
dc.identifier.wosidWOS:000466308400048
dc.issue.numero5
dc.language.isoen
dc.pagina.final2463
dc.pagina.inicio2455
dc.revistaClinical oral investigations
dc.rightsacceso restringido
dc.subjectPlatelet-rich plasma
dc.subjectPeriodontal ligament
dc.subjectMesenchymal stem cells
dc.subjectSerum-free culture media
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleComparative effect of platelet-rich plasma, platelet-poor plasma, and fetal bovine serum on the proliferative response of periodontal ligament cell subpopulations
dc.typeartículo
dc.volumen23
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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