A Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis

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dc.contributor.authorRoschzttardtz, Hannetz
dc.contributor.authorFuentes, Ignacia
dc.contributor.authorVasquez, Marcos
dc.contributor.authorCorvalan, Claudia
dc.contributor.authorLeon, Gabriel
dc.contributor.authorGomez, Isabel
dc.contributor.authorAraya, Alejandro
dc.contributor.authorHoluigue, Loreto
dc.contributor.authorVicente Carbajosa, Jesus
dc.contributor.authorJordana, Xavier
dc.date.accessioned2024-01-10T13:11:09Z
dc.date.available2024-01-10T13:11:09Z
dc.date.issued2009
dc.description.abstractMitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here, we present data demonstrating that three abscisic acid-responsive elements and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the abscisic acid-responsive elements are shown to be a target for bZIP53, a member of the basic leucine zipper (bZIP) family of transcription factors. We show that group S1 bZIP53 protein binds the promoter as a heterodimer with group C bZIP10 or bZIP25. To the best of our knowledge, the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.
dc.description.funderFondecyt-Chile
dc.description.funderMillennium Nucleus for Plant Functional Genomics, Millennium Scientific Initiative Program, Mideplan, Chile
dc.description.funderAECI-Spain
dc.fechaingreso.objetodigital2024-05-23
dc.format.extent12 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1104/pp.109.136531
dc.identifier.eissn1532-2548
dc.identifier.issn0032-0889
dc.identifier.pubmedidMEDLINE:19261733
dc.identifier.urihttps://doi.org/10.1104/pp.109.136531
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/78004
dc.identifier.wosidWOS:000265661700008
dc.information.autorucCiencias Biológicas;Corvalan C ;S/I;142372
dc.information.autorucCiencias Biológicas;Holuigue L;S/I;50349
dc.information.autorucCiencias Biológicas;Jordana X ;S/I;99673
dc.information.autorucMedicina;Leon G ;S/I;1001938
dc.information.autorucCiencias Biológicas;Roschzttardtz H ;S/I;134728
dc.information.autorucCiencias Biológicas;Vasquez M ;S/I;132624
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final95
dc.pagina.inicio84
dc.publisherAMER SOC PLANT BIOLOGISTS
dc.revistaPLANT PHYSIOLOGY
dc.rightsacceso abierto
dc.subjectEMBRYOGENESIS ABUNDANT PROTEIN
dc.subjectCYTOCHROME-C-OXIDASE
dc.subjectSUCCINATE-DEHYDROGENASE
dc.subjectPROLINE DEHYDROGENASE
dc.subjectREDUCED EXPRESSION
dc.subjectTHALIANA
dc.subjectABI3
dc.subjectPROMOTER
dc.subjectMATURATION
dc.subjectHETERODIMERIZATION
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleA Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis
dc.typeartículo
dc.volumen150
sipa.codpersvinculados142372
sipa.codpersvinculados50349
sipa.codpersvinculados99673
sipa.codpersvinculados1001938
sipa.codpersvinculados134728
sipa.codpersvinculados132624
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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