In vitro time-kill curves study of three antituberculous combinations against <i>Mycobacterium tuberculosis</i> clinical isolates

dc.contributor.authorLopez-Gavin, Alexandre
dc.contributor.authorTudo, Griselda
dc.contributor.authorRey-Jurado, Emma
dc.contributor.authorVergara, Andrea
dc.contributor.authorHurtado, Juan Carlos
dc.contributor.authorGonzalez-Martin, Julian
dc.date.accessioned2025-01-23T21:32:10Z
dc.date.available2025-01-23T21:32:10Z
dc.date.issued2016
dc.description.abstractThe objective of this study was to examine the in vitro synergism of three-drug combinations against Mycobacterium tuberculosis (levofloxacin/linezolid/ethambutol, levofloxacin/amikacin/ethambutol and levofloxacin/linezolid/amikacin) using the time-kill curves method. In total, 8 multidrug-resistant and 12 drug-susceptible M. tuberculosis isolates were used. Minimum inhibitory concentrations (MICs) of the isolates for each drug were determined by the proportions method. Time-kill curves were studied for the three combinations proposed over 14 days using two different protocols. In protocol 1, 0.5 x MIC for each drug was used. In protocol 2, 0.5 x MIC for levofloxacin and linezolid and 0.25 x MIC for amikacin and ethambutol were used. The MICs for all of the isolates studied were 0.5 mg/L for levofloxacin and linezolid and 2.5 mg/L for ethambutol and amikacin. All of the combinations displayed an additive activity compared with the most active individual drug. In conclusion, these results demonstrate that the three combinations tested were equally effective against M. tuberculosis isolates. The study of antituberculous combinations using in vitro methods is an excellent first step to predict their effect in clinical development phases as well as to test new regimens of the antituberculous drugs currently available. (C) 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.ijantimicag.2015.10.016
dc.identifier.eissn1872-7913
dc.identifier.issn0924-8579
dc.identifier.urihttps://doi.org/10.1016/j.ijantimicag.2015.10.016
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101485
dc.identifier.wosidWOS:000368040400015
dc.issue.numero1
dc.language.isoen
dc.pagina.final100
dc.pagina.inicio97
dc.revistaInternational journal of antimicrobial agents
dc.rightsacceso restringido
dc.subjectTime-kill
dc.subjectTuberculosis
dc.subjectAntituberculous drugs
dc.subjectCombinations
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIn vitro time-kill curves study of three antituberculous combinations against <i>Mycobacterium tuberculosis</i> clinical isolates
dc.typeartículo
dc.volumen47
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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