At least two receptors of asymmetric acetylcholinesterase are present at the synaptic basal lamina of <i>Torpedo</i> electric organ

Simple item page
dc.contributor.authorCasanueva, OI
dc.contributor.authorDeprez, P
dc.contributor.authorGarcía-Huidobro, T
dc.contributor.authorInestrosa, NC
dc.date.accessioned2025-01-21T01:32:30Z
dc.date.available2025-01-21T01:32:30Z
dc.date.issued1998
dc.description.abstractAsymmetric acetylcholinesterase (AChE) is anchored to the basal lamina (BL) of cholinergic synapses via its collagenic tail, yet the complement of matrix receptors involved in its attachment remains unknown. The development of a novel overlay technique has allowed us to identify two Torpedo BL components that bind asymmetric AChE: a polypeptide of similar to 140kDa and a doublet of 195-215kDa. These were found to stain metachromatically with Coomassie blue R-250, mere solubilized by acetic acid, and were sensitive to collagenase treatment. Upon sequence analysis, the 140kDa polypeptide yielded a characteristic collagenous motif. Another AChE-binding BL constituent, identified by overlay, corresponded to a heparan sulfate proteoglycan. Lastly, we established that this proteoglycan, but not the collagenous proteins, interacted with at least one heparin binding domain of the collagenic tail of AChE. Our results indicate that at least two BL receptors are likely to exist for asymmetric AChE in Torpedo electric organ. (C) 1998 Academic Press.
dc.fuente.origenWOS
dc.identifier.issn0006-291X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97274
dc.identifier.wosidWOS:000076088000022
dc.issue.numero2
dc.language.isoen
dc.pagina.final317
dc.pagina.inicio312
dc.revistaBiochemical and biophysical research communications
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAt least two receptors of asymmetric acetylcholinesterase are present at the synaptic basal lamina of <i>Torpedo</i> electric organ
dc.typeartículo
dc.volumen250
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas