EFFECT OF CASTRATION AND TESTOSTERONE ON NOREPINEPHRINE STORAGE AND ON THE RELEASE OF [H-3]NOREPINEPHRINE FROM RAT VAS-DEFERENS

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dc.contributor.authorLARA, H
dc.contributor.authorGALLEGUILLOS, X
dc.contributor.authorARRAU, J
dc.contributor.authorBELMAR, J
dc.date.accessioned2025-01-23T19:27:41Z
dc.date.available2025-01-23T19:27:41Z
dc.date.issued1985
dc.description.abstractNorepinephrine and dopamine-.beta.-hydroxylase, used as noradrenergic vesicle markers, were decreased in the rat vas deferens 10 days after castration. Five days of testosterone administration to castrated animals increased the enzyme activity over that of controls but did not modify norepinephrine content. In tissue fractions obtained by differential centrifugation, the highest activities of the noradrenergic markers appeared in the vesicular fraction of controls and in the soluble fraction of castrated animals. Testosterone reversed the effect of castration: it increased dopamine-.beta.-hydroxylase activity in the vesicular and soluble fractions, while norepinephrine increased only in the vesicular fraction. Results obtained after continuous sucrose gradient centrifugation of vesicular fractions suggested that these changes principally affected the number of light noradrenergic vesicles while testosterone increased the number of vesicles reduced by castration. Hormonal manipulations also modified some functional properties of nerve endings: norepinephrine depletion after transmural stimulation in the presence of tetraethylammonium, as well as the release of the neurotransmitter, were decreased after castration. These effects were reversed by testosterone. A modulatory effect of testosterone on the norepinephrine storage system and on the functional properties of the adrenergic innervation of vas deferns is suggested.
dc.fuente.origenWOS
dc.identifier.eissn1872-9754
dc.identifier.issn0197-0186
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99756
dc.identifier.wosidWOS:A1985AMM1300015
dc.issue.numero4
dc.language.isoen
dc.pagina.final674
dc.pagina.inicio667
dc.revistaNeurochemistry international
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEFFECT OF CASTRATION AND TESTOSTERONE ON NOREPINEPHRINE STORAGE AND ON THE RELEASE OF [H-3]NOREPINEPHRINE FROM RAT VAS-DEFERENS
dc.typeartículo
dc.volumen7
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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