Allopregnanolone-induced modification of presynaptic basal and K<SUP>+</SUP>-induced [<SUP>3</SUP>H]-norepinephrine efflux from rat cortical slices during the estrous cycle

Simple item page
dc.contributor.authorBelmar, J
dc.contributor.authorCuellar, C
dc.contributor.authorLlona, I
dc.contributor.authorArancibia, S
dc.contributor.authorKusch, C
dc.contributor.authorTapia-Arancibia, L
dc.contributor.authorPinter, A
dc.contributor.authorPerez, H
dc.date.accessioned2025-01-21T01:32:30Z
dc.date.available2025-01-21T01:32:30Z
dc.date.issued1998
dc.description.abstractSuperfused frontal slices of cerebral cortex were preloaded with [H-3]-norepinephrine ([H-3]NE). Basal [H-3]NE efflux and Kf-induced [H-3]NE release were studied during the estrous cycle and in the presence of neurosteroids. Basal [H-3]NE efflux showed estrous cycle-related variations, with lowest values found during estrus and diestrus II. Allopregnanolone (10(-9) M) potentiated basal [H-3]NE efflux from the Ist minute of its application; the effect of the steroid was still present after 20 min. This effect was also dependent upon the estrous cycle, since basal [H-3]NE efflux was mainly increased during estrus diestrus I, and to a lesser degree only during proestrus. During diestrus II and after ovariectomy, basal [H-3]NE efflux was no longer affected by the neurosteroid. In the presence of yohimbine (10(-6) M), the effect of allopregnanolone on basal efflux was potentiated only during the first 3 min but vanished thereafter. Allopregnanolone (10(-9) M) potentiated the K+-induced [H-3]NE release during estrus, but pregnenolone (10(-9) M) was ineffective, suggesting specificity of the neurosteroid. Yohimbine (10(-6) M) also potentiated K+-induced [H-3]NE release. When applied simultaneously with allopregnanolone (10(-9) M), a potentiating effect on [H-3]NE release was observed. The present results suggest that allopregnanolone is a neurosteroid able to modulate norepinephrine release in the cerebral cortex in an estrous cycle-dependent manner, and that the effect could involve noradrenergic alpha-2 receptors.
dc.fuente.origenWOS
dc.identifier.eissn1423-0194
dc.identifier.issn0028-3835
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97272
dc.identifier.wosidWOS:000076564700005
dc.issue.numero4
dc.language.isoen
dc.pagina.final271
dc.pagina.inicio264
dc.revistaNeuroendocrinology
dc.rightsacceso restringido
dc.subjectcatecholamines
dc.subjectcerebral cortex
dc.subjectallopregnanolone
dc.subjectneurosteroids
dc.subjectestrous cycle
dc.titleAllopregnanolone-induced modification of presynaptic basal and K<SUP>+</SUP>-induced [<SUP>3</SUP>H]-norepinephrine efflux from rat cortical slices during the estrous cycle
dc.typeartículo
dc.volumen68
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas