THE ACROSOME REACTION-INDUCING ACTIVITY OF INDIVIDUAL HUMAN FOLLICULAR-FLUID SAMPLES IS HIGHLY VARIABLE AND IS RELATED TO THE STEROID CONTENT

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dc.contributor.authorMORALES, P
dc.contributor.authorLLANOS, M
dc.contributor.authorGUTIERREZ, G
dc.contributor.authorKOHEN, P
dc.contributor.authorVIGIL, P
dc.contributor.authorVANTMAN, D
dc.date.accessioned2025-01-23T19:22:11Z
dc.date.available2025-01-23T19:22:11Z
dc.date.issued1992
dc.description.abstractIn this study, we have evaluated the relationship between the acrosome reaction-inducing activity of individual human follicular fluid samples and their steroid content. Eighteen samples of follicular fluid were obtained during egg retrieval in six patients undergoing assisted fertilization. Motile spermatozoa were incubated in modified Tyrode's medium (26 mg/ml bovine serum albumin) for 20 h at 1 X 10(7) cells/ml. In a single experiment, aliquots of a semen specimen were simultaneously treated with an aliquot of each follicular fluid sample. The percentage of acrosome reacted spermatozoa was determined using fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC - PSA) lectin. The fluids were also analysed by radioinimunoassay to determine the levels of progesterone, 17-alpha-hydroxy-progesterone, testosterone and oestradiol. The results showed that there was a positive, highly significant correlation between the acrosome reaction-inducing activity and the progesterone level of each follicular fluid sample (r = 0.72, P < 0.005). Additionally, treatment of the follicular fluid samples with charcoal-dextran caused both a decrease in progesterone concentration and the total loss of the acrosome reaction-inducing activity. The addition of progesterone restored the acrosome reaction-inducing ability in 88% of samples. These data support the idea that progesterone in follicular fluid is the molecule responsible for inducing the acrosome reaction in human spermatozoa.
dc.fuente.origenWOS
dc.identifier.issn0268-1161
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98984
dc.identifier.wosidWOS:A1992HY55600014
dc.issue.numero5
dc.language.isoen
dc.pagina.final651
dc.pagina.inicio646
dc.revistaHuman reproduction
dc.rightsacceso restringido
dc.subjectACROSOME
dc.subjectFERTILIZATION
dc.subjectHUMAN SPERMATOZOA
dc.subjectPROGESTERONE
dc.subjectSTEROIDS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTHE ACROSOME REACTION-INDUCING ACTIVITY OF INDIVIDUAL HUMAN FOLLICULAR-FLUID SAMPLES IS HIGHLY VARIABLE AND IS RELATED TO THE STEROID CONTENT
dc.typeartículo
dc.volumen7
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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